COPPER-CHELATES AS PROBES OF BIOLOGICAL-SYSTEMS - STABLE COPPER-COMPLEXES WITH A MACROCYCLIC BIFUNCTIONAL CHELATING AGENT
COPPER-CHELATES AS PROBES OF BIOLOGICAL-SYSTEMS - STABLE COPPER-COMPLEXES WITH A MACROCYCLIC BIFUNCTIONAL CHELATING AGENT
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DOI：
10.1016/0003-2697(85)90653-0
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
DENARDO, SJ
中科院分区：
文献类型：
--
作者：
MOI, MK;MEARES, CF;DENARDO, SJ
用螯合金属离子标记的单克隆抗体具有许多潜在的应用。合成了一种新的双功能金属螯合剂6-（对溴乙酰胺基苄基）-1，4，8，11-四氮杂环十四烷-N，N“，N”"，N“-四乙酸，它能与蛋白质共价结合，在生理条件下能与人血清中的铜稳定结合。相比之下，基于EDTA或二亚乙基三胺五乙酸的其他试剂在相同条件下迅速将Cu损失至血清白蛋白。
Monoclonal antibodies tagged with chelated metal ions have numerous potential applications. The synthesis of a new bifunctional metal chelator, 6(p-bromoacetamidobenzyl)-1,4,8,11-tetraazacyclotetradecane-N,N'',N'''',N''''''-tetraacetic acid, which can be covalently attached to proteins and which binds Cu stably in human serum under physiological conditions. In contrast, other reagents based on EDTA or diethylenetriaminepentaacetic acid rapidly lose Cu to serum albumin under the same conditions.