NOVEL METHOD FOR DETECTION OF BETA-LACTAMASES BY USING A CHROMOGENIC CEPHALOSPORIN SUBSTRATE
NOVEL METHOD FOR DETECTION OF BETA-LACTAMASES BY USING A CHROMOGENIC CEPHALOSPORIN SUBSTRATE
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DOI：
10.1128/aac.1.4.283
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发表时间：
1972-01-01
影响因子：
4.9
通讯作者：
MORRIS, A
中科院分区：
文献类型：
--
作者：
OCALLAGHAN, CH;SHINGLER, AH;MORRIS, A
一种具有高活性β-内酰胺环的新型头孢菌素在许多细菌（包括葡萄球菌、芽孢杆菌、肠杆菌科和假单胞菌）的β-内酰胺酶存在下能立即变色。该反应仅限于产生β-内酰胺酶的生物，但它足够敏感，表明在以前认为不产生β-内酰胺酶的菌株中存在少量这种酶。该化合物具有不同寻常的紫外光谱，可以通过测量380- 500 nm区域的吸收变化来定量地跟踪颜色变化，而头孢菌素通常在该区域没有吸收。颜色的发展被认为是β-内酰胺环与3取代基异常高度共轭的结果。虽然在细菌中只有β-内酰胺酶会产生这种颜色变化，但实验动物的血清和组织也会迅速产生这种颜色的分解产物，然后随尿液排出体外。哺乳动物分解的机制被认为与在细菌中发现的不同。
A new cephalosporin with a highly reactive β-lactam ring was found to give an immediate color change in the presence of β-lactamases from many bacteria, including staphylococci,Bacillusspecies,Enterobacteriaceae, andPseudomonas. The reaction is confined to organisms producing β-lactamases, but it is sufficiently sensitive to indicate the presence of this enzyme is small amounts in strains previously considered not to produce it. The compound has an unusual ultraviolet spectrum, and the color change can be followed quantitatively by measuring changes in absorption which occur in the 380- to 500-nm region, where cephalosporins normally have no absorption. The development of color is thought to be a consequence of the β-lactam ring being unusually highly conjugated with the 3-substituent. Although in the bacteria only β-lactamases produce this color change, it was found that serum and tissues from experimental animals also rapidly produced the colored breakdown product, which was then excreted in the urine. The mechanism of the mammalian breakdown was considered to be different from that found in bacteria.