Quantitative Evaluation of Mechanosensing of Cells on Dynamically Tunable Hydrogels
Quantitative Evaluation of Mechanosensing of Cells on Dynamically Tunable Hydrogels
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DOI：
10.1021/ja1060615
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发表时间：
2011-02-09
影响因子：
15
通讯作者：
Tanaka, Motomu
中科院分区：
文献类型：
--
作者：
Yoshikawa, Hiroshi Y.;Rossetti, Fernanda F.;Tanaka, Motomu
基于ABA三嵌段共聚物凝胶剂的水凝胶薄膜[其中A是pH敏感的聚(甲基丙烯酸二异丙氨基)甲基丙烯酸乙酯(PDPA)，B是生物相容的聚(甲基丙烯酰氧基)乙基磷酰胆碱(PMPC)]被用作刺激响应底物，允许微调小鼠成肌细胞所经历的力学环境。通过仔细调节pH，水凝胶膜的弹性可以可逆地调节到40倍，而不会对细胞活力产生不利影响。成肌细胞在增加水凝胶弹性的过程中表现出明显的应力纤维形成和扁平化。作为一种评估细胞黏附强度的新工具，我们将皮秒激光与倒置显微镜相结合，并利用激光脉冲产生的强烈冲击波来确定细胞分离所需的临界压力。此外，我们证明了水凝胶弹性的突然跳跃可以用来监测细胞如何使其形态适应其机械环境的变化。
Thin hydrogel films based on an ABA triblock copolymer gelator [where A is pH-sensitive poly(2-(diisopropylamino)ethyl methacrylate) (PDPA) and B is biocompatible poly(2-(methacryloyloxy)ethyl phosphorylcholine) (PMPC)] were used as a stimulus-responsive substrate that allows fine adjustment of the mechanical environment experienced by mouse myoblast cells. The hydrogel film elasticity could be reversibly modulated by a factor of 40 via careful pH adjustment without adversely affecting cell viability. Myoblast cells exhibited pronounced stress fiber formation and flattening on increasing the hydrogel elasticity. As a new tool to evaluate the strength of cell adhesion, we combined a picosecond laser with an inverted microscope and utilized the strong shock wave created by the laser pulse to determine the critical pressure required for cell detachment. Furthermore, we demonstrate that an abrupt jump in the hydrogel elasticity can be utilized to monitor how cells adapt their morphology to changes in their mechanical environment.