Designing of Collagen Based Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Scaffolds for Tissue Engineering
Designing of Collagen Based Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Scaffolds for Tissue Engineering
复制标题
用于组织工程的基于胶原的聚（3-羟基丁酸酯-co-4-羟基丁酸酯）支架的设计
DOI：
--
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
A. Amirul
中科院分区：
文献类型：
--
作者：
S. Vigneswari;H. Khalil;A. Amirul
采用双溶剂体系，用胶原蛋白对P（3 HB-co-4 HB）共聚物进行改性。采用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、有机元素分析（CHN）和水接触角测量等方法对样品的表面性质进行了表征。胶原蛋白浓度，支架厚度，和4 HB的摩尔分数对亲水性的影响进行了优化，通过田口方法。采用正交试验法对亲水性支架材料的响应进行了研究。使用方差分析（ANOVA）确定显著性参数并确定每个参数的最佳水平。结果还表明，P（3 HB-co-4 HB）/胶原蛋白共混物支架的亲水性随着胶原蛋白浓度增加到15重量%，摩尔分数为50摩尔%，在0.1 mm的支架厚度。通过成纤维细胞（L929）培养评价P（3 HB-co-4 HB）/胶原共混物表面的生物相容性。与P（3 HB-co-4 HB）共聚物支架相比，胶原共混物支架表面显示出显著的细胞粘附和生长。
P(3HB-co-4HB) copolymer was modified using collagen by adapting dual solvent system. The surface properties of samples were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), organic elemental analysis (CHN analysis), and water contact angle measurements. The effects of collagen concentration, scaffold thickness, and 4HB molar fraction on the hydrophilicity were optimized by the Taguchi method. The orthogonal array experiment was conducted to obtain the response for a hydrophilic scaffold. Analysis of variance (ANOVA) was used to determine the significant parameters and determine the optimal level for each parameter. The results also showed that the hydrophilicity of P(3HB-co-4HB)/collagen blend scaffolds increased as the collagen concentration increased up to 15 wt% with a molar fraction of 50 mol% at 0.1 mm scaffold thickness. The biocompatibility of the P(3HB-co-4HB)/collagen blend surface was evaluated by fibroblast cell (L929) culture. The collagen blend scaffold surfaces showed significant cell adhesion and growth as compared to P(3HB-co-4HB) copolymer scaffolds.