The role of Hox genes during vertebrate limb development
The role of Hox genes during vertebrate limb development
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DOI：
10.1016/j.gde.2007.05.011
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发表时间：
2007-08-01
影响因子：
4
通讯作者：
Duboule, Denis
中科院分区：
文献类型：
--
作者：
Zakany, Jozsef;Duboule, Denis
Hox基因在脊椎动物肢体发育中的潜在作用通过小鼠的基因表达分析而引起关注（P多勒，JC lzpisua-Belmonte，H Falkenstein，A Renucci，D Duboule，Nature 1989，342：767-772），当时肢体生长和模式被认为取决于两个不同的且相当独立的坐标系;一个用于前-后轴，另一个用于近-远轴（参见D Duboule，P多勒，EMBO J 1989，8：1497-1505）。在过去的几年里，这些基因的功能和调节已经解决了使用鸡和小鼠的功能获得和功能丧失的方法。使用顺式构型、反式构型或顺式/反式构型的多个突变已经证实Hox基因对于适当的肢体发育是必不可少的，其中它们参与结构的生长和组织。尽管它们的分子作用机制仍然有些难以捉摸，但这些广泛的遗传分析的结果证实，在肢体的发育过程中，不能孤立地考虑各个轴，并且对肢体发育的更全面的看法应该优于对这些复杂结构的简单的腕足，国际象棋网格般的方法。考虑到这一点，现在可以重新评估Hox基因在肢体生长和发育过程中的功能输入。
The potential role of Hox genes during vertebrate limb development was brought into focus by gene expression analyses in mice (P Dolle, JC lzpisua-Belmonte, H Falkenstein, A Renucci, D Duboule, Nature 1989, 342:767-772), at a time when limb growth and patterning were thought to depend upon two distinct and rather independent systems of coordinates; one for the anterior-to-posterior axis and the other for the proximal-to-distal axis (see D Duboule, P Dolle, EMBO J 1989, 8:1497-1505). Over the past years, the function and regulation of these genes have been addressed using both gain-of-function and loss-of-function approaches in chick and mice. The use of multiple mutations either in cis-configuration in trans-configuration or in cis/trans configurations, has confirmed that Hox genes are essential for proper limb development, where they participate in both the growth and organization of the structures. Even though their molecular mechanisms of action remain somewhat elusive, the results of these extensive genetic analyses confirm that, during the development of the limbs, the various axes cannot be considered in isolation from each other and that a more holistic view of limb development should prevail over a simple cartesian, chess grid-like approach of these complex structures. With this in mind, the functional input of Hox genes during limb growth and development can now be re-assessed.