Calcium transients in mammalian ventricular muscle.
Calcium transients in mammalian ventricular muscle.
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哺乳动物心室肌中的钙瞬变。
DOI：
10.1093/eurheartj/1.suppl_1.5
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发表时间：
1980
影响因子：
39.3
通讯作者：
Kurihara,S
中科院分区：
文献类型：
--
作者：
Allen,DG;Kurihara,S
将钙敏感的光蛋白Aequorin显微注射到大鼠和猫的心肌细胞中。在随后的肌肉刺激过程中，可以检测到光发射，该信号是细胞内[Ca++]的函数。描述了收缩过程中细胞内[Ca~(++)]瞬变的时间过程和幅度。本文描述了改变外界[Ca~(++)]和刺激频率以及在浴液中加入肾上腺素和咖啡因对张力和发光的影响。这些结果表明，外部钙和刺激频率的变化通过改变细胞内[Ca++]来改变张力，而肾上腺素还改变了收缩系统对细胞内[Ca++]的敏感性。结果还表明，尽管细胞内[Ca++]的下降总是先于松弛，但[Ca++]下降的时间进程通常不是松弛过程中的限速步骤。
The calcium-sensitive photoprotein aequorin was microinjected into cells of rat and cat ventricular muscle. During subsequent stimulation of the muscle light emission could be detected and this signal is a function of the intracellular [Ca++]. The time course and amplitude of the intracellular [Ca++] transient occurring during contraction is described. The effects on tension and light emission of changing external [Ca++] and stimulus frequency and of adding adrenaline and caffeine to the bathing solution are described. These results show that changes in external calcium and stimulus frequency alter tension by means of changes in the intracellular [Ca++] whereas adrenaline in addition alters the sensitivity of the contractile system to intracellular [Ca++]. The results also suggest that although a fall in intracellular [Ca++] always precedes relaxation, the time course of the fall of [Ca++] is not generally a rate limiting step in the lime course of relaxation.