Dynamic behavior of liquid droplets with enzyme compartmentalization triggered by sequential glycolytic enzyme reactions
Dynamic behavior of liquid droplets with enzyme compartmentalization triggered by sequential glycolytic enzyme reactions
复制标题
连续糖酵解酶反应触发酶区室化的液滴动态行为
DOI：
10.1039/d1cc04596b
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发表时间：
2021
影响因子：
4.9
通讯作者：
Shiraki Kentaro
中科院分区：
文献类型：
--
作者：
Ura Tomoto;Tomita Shunsuke;Shiraki Kentaro
通过液-液相分离（LLPS）形成的动态液滴被认为参与了多种生物过程的调节。在这里，建立了一个模型LLPS系统，耦合了一个顺序的糖酵解酶反应，以重现液滴的动态控制；(1)由聚l -赖氨酸和核苷酸组成的液滴分别分隔了两种不同的酶（己糖激酶和葡萄糖-6-磷酸脱氢酶），加速了整个反应；(2)每个酶反应通过转化支架核苷酸触发液滴的形成、溶解和长期保留。该模型系统将提供与活细胞中LLPS相关的酶的新方面。
Dynamic droplet formation via liquid–liquid phase separation (LLPS) is believed to be involved in the regulation of various biological processes. Here, a model LLPS system coupled with a sequential glycolytic enzymatic reaction was developed to reproduce the dynamic control of liquid droplets; (i) the droplets, which consist of poly-L-lysine and nucleotides, compartmentalize two different enzymes (hexokinase and glucose-6-phosphate dehydrogenase) individually, accelerating the overall reaction, and (ii) each enzymatic reaction triggers the formation, dissolution and long-term retention of the droplets by converting the scaffold nucleotides. This model system will offer a new aspect of enzymes associated with LLPS in living cells.