Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus.
Metabolic Profiles in Cell Lines Infected with Classical Swine Fever Virus.
复制标题
感染猪瘟病毒的细胞系的代谢特征
DOI：
10.3389/fmicb.2017.00691
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发表时间：
2017
影响因子：
5.2
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Gou H;Zhao M;Yuan J;Xu H;Ding H;Chen J
病毒需要能量和来自宿主细胞的生物合成前体来进行复制。了解猪瘟病毒（CSFV）与宿主细胞之间的代谢相互作用，对于探索猪瘟（CSF）复杂的病理机制具有重要意义。在本研究中，我们首次采用气相色谱-质谱联用（GC-MS）方法检测感染CSFV的PK-15和3D4/2细胞的代谢谱。CSFV感染引起的PK-15细胞差异代谢产物主要包括糖酵解过程中葡萄糖6-磷酸[fold change (FC) =−1.94)]和甘油醛-3-磷酸（FC =−1.83）水平降低，戊糖磷酸途径中核酮糖5-磷酸（FC =−1.51），嘌呤生物合成过程中鸟苷（FC =−1.24）和肌苷（FC =−1.16）水平降低，而柠檬酸循环过程中2-酮异戊酸（FC = 0.63）水平升高。鸟氨酸（FC = 0.56）和脯氨酸（FC = 0.62）在精氨酸和脯氨酸代谢中的作用。然而，CSFV感染引起3D4/2细胞代谢物的变化包括糖酵解过程中甘油醛-3-磷酸（FC = - 0.77）和丙酮酸（FC = - 1.42）的降低，柠檬酸循环过程中2-酮戊二酸（FC = - 1.52）的降低，嘧啶代谢过程中胞嘧啶（FC = 2.15）的升高。我们的数据显示CSFV可能重建细胞代谢程序，从而帮助病毒复制。这些发现可能对开发新的生物标志物靶标，用于诊断和鉴定酶抑制剂或代谢物作为抗病毒药物，或筛选病毒基因产物作为疫苗具有重要意义。
Viruses require energy and biosynthetic precursors from host cells for replication. An understanding of the metabolic interplay between classical swine fever virus (CSFV) and host cells is important for exploring the complex pathological mechanisms of classical swine fever (CSF). In the current study, and for the first time, we utilized an approach involving gas chromatography coupled with mass spectrometry (GC-MS) to examine the metabolic profiles within PK-15 and 3D4/2 cells infected with CSFV. The differential metabolites of PK-15 cells caused by CSFV infection mainly included the decreased levels of glucose 6-phosphate [fold change (FC) = −1.94)] and glyceraldehyde-3-phosphate (FC = −1.83) during glycolysis, ribulose 5-phosphate (FC = −1.51) in the pentose phosphate pathway, guanosine (FC = −1.24) and inosine (FC = −1.16) during purine biosynthesis, but the increased levels of 2-ketoisovaleric acid (FC = 0.63) during the citrate cycle, and ornithine (FC = 0.56) and proline (FC = 0.62) during arginine and proline metabolism. However, metabolite changes caused by CSFV infection in 3D4/2 cells included the reduced glyceraldehyde-3-phosphate (FC = −0.77) and pyruvic acid (FC = −1.42) during glycolysis, 2-ketoglutaric acid (FC = −1.52) in the citrate cycle, and the elevated cytosine (FC = 2.15) during pyrimidine metabolism. Our data showed that CSFV might rebuild cellular metabolic programs, thus aiding viral replication. These findings may be important in developing targets for new biomarkers for the diagnosis and identification of enzyme inhibitors or metabolites as antiviral drugs, or screening viral gene products as vaccines.