GENETIC-ANALYSIS AND THE CONSTRUCTION OF MASTER STRAINS FOR ASSIGNMENT OF GENES TO 6 LINKAGE GROUPS IN ASPERGILLUS-NIGER
GENETIC-ANALYSIS AND THE CONSTRUCTION OF MASTER STRAINS FOR ASSIGNMENT OF GENES TO 6 LINKAGE GROUPS IN ASPERGILLUS-NIGER
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DOI：
10.1007/bf00521266
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发表时间：
1988-11-01
期刊：
影响因子：
2.5
通讯作者：
SLAKHORST, SM
中科院分区：
文献类型：
--
作者：
BOS, CJ;DEBETS, AJM;SLAKHORST, SM
已经开始建立一个收集尼日尔颜色和营养缺陷型突变体的同基因背景作为遗传标记的来源。所有菌株都具有短分生孢子梗（cspA 1），这使得它们易于在测试板上处理。通过体细胞重组逐步组合遗传标记。从异核体中收集分生孢子，以10-6-10-5的频率获得体细胞二倍体。苯菌灵诱导杂合二倍体的单倍体化。对于非连锁标记，重组频率在35%-65%之间。低频率的重组体被发现之间的标记在同一条染色体上，但这有时被打乱的有丝分裂交换在早期阶段的二倍体。构建了具有六个连锁群的标记的主菌株。
A start has been made on establishing a collection of Aspergillus niger colour and auxotrophic mutants with an isogenic background for use as a source of genetic markers. All strains have short conidiophores (cspA1), which makes them easy to handle on test plates. Genetic markers were combined stepwise by somatic recombination. Somatic diploids were obtained at frequencies of 10-6-10-5 with conidiospores collected from a heterokaryon. The haploidization of heterozygous diploids was induced by benomyl. For unlinked markers, the frequency of recombinants varied from 35%-65%. Low frequencies of recombinants were found between markers on a same chromosome, but this was sometimes disturbed by mitotic crossing-over during an early stage of the diploid. Master strains were constructed having markers for six linkage groups.