Usefulness of human papillomavirus detection in oral rinse as a biomarker of oropharyngeal cancer
Usefulness of human papillomavirus detection in oral rinse as a biomarker of oropharyngeal cancer
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DOI：
10.1080/00016489.2016.1274426
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发表时间：
2017-01-01
影响因子：
1.4
通讯作者：
Yoshizaki, Tomokazu
中科院分区：
文献类型：
--
作者：
Yoshida, Hiroshi;Murono, Shigeyuki;Yoshizaki, Tomokazu
结论：自动巢式GP 5 +/GP 6 +PCR检测口腔冲洗液中的人乳头瘤病毒（HPV）-DNA可作为口咽癌（oropharyngealcancer）的生物标志物。背景：本研究旨在确定口腔冲洗液检测HPV-DNA作为HPV阳性口咽癌（OPC）的生物标志物的有用性。收集口腔冲洗液和扁桃体拭子，并进行HPV-DNA的自动巢式GP 5 +/GP 6 +PCR。结果：口咽癌患者口腔冲洗液和扁桃体拭子中HPV-DNA检出率明显高于非口咽癌患者和非癌性疾病患者。在12例p16阳性的OPC病例中，有9例在口腔冲洗液中检测到HPV-DNA，而p16阴性的OPC病例均未检测到HPV-DNA。所有p16阳性病例的肿瘤组织中HPV-DNA均阳性。以p16免疫组化为基础，检测漱口液中HPV-DNA的敏感性为75%，特异性为100%。在诊断时口腔冲洗液中HPV-DNA阳性的9例可评估OPC病例中，有8例在治疗后的7例口腔冲洗液中检测不到HPV-DNA。
Conclusion: The detection of human papillomavirus (HPV)-DNA in oral rinse with auto-nested GP5+/GP6+PCR is useful as a biomarker of oropharyngeal cancer.Background: This study aimed to determine the usefulness of oral rinse to detect HPV-DNA as a biomarker of HPV-positive oropharyngeal cancer (OPC).Patients and methods: One hundred and ten patients with various head and neck diseases, including 19 patients with OPC, were enrolled. Oral rinse and tonsillar swab were collected, and auto-nested GP5+/GP6+PCR for HPV-DNA was performed. For oropharyngeal cancer, p16 immunostaining was also conducted.Results: The rate of HPV-DNA detection in both oral rinse and tonsillar swab was significantly higher in OPC compared with non-OPC upper respiratory tract cancer and non-cancer diseases. HPV-DNA was detected in oral rinse in nine out of 12 p16-positive OPC cases, while none of the p16-negative OPC cases demonstrated detectable HPV-DNA. All p16-positive cases were also positive for HPV-DNA in tumor tissue. Based on p16 immunostaining, the sensitivity and specificity of HPV-DNA detection in oral rinse were 75% and 100%, respectively. Among eight of nine evaluable OPC cases positive for HPV-DNA in oral rinse at diagnosis, HPV-DNA was undetectable in oral rinse in seven cases after treatment.