Circular RNA Expression Profiles Alter Significantly after Traumatic Brain Injury in Rats
Circular RNA Expression Profiles Alter Significantly after Traumatic Brain Injury in Rats
复制标题
大鼠脑外伤后环状 RNA 表达谱显着改变
DOI：
10.1089/neu.2017.5468
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发表时间：
2018-04-12
影响因子：
4.2
通讯作者：
Jiang, Ji-yao
中科院分区：
文献类型：
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作者：
Xie, Bao-shu;Wang, Yi-qin;Jiang, Ji-yao
环状RNA（circRNA）参与多种疾病。然而，circRNA在创伤性脑损伤（TBI）中的作用仍然未知。在这项研究中，使用circRNA微阵列来分析TBI后大鼠海马中改变的circRNA。观察到总共192个circRNA在TBI后差异表达（倍数变化[FC] >= 1.5和p < 0.05），包括98个上调和94个下调。基因本体论（GO）和京都基因和基因组百科全书（KEGG）通路分析表明，许多从改变的circRNA的宿主基因转录的信使RNA（mRNA）与脑损伤和神经再生有关。使用基于TargetScan和米兰达的Arraystar自制miRNA靶标预测软件预测CircRNA/microRNA（miRNA）相互作用。因此，我们的研究表明，TBI后大鼠海马circRNA表达模式的改变，这可能在TBI后的生理和病理过程中发挥重要作用。这些发现不仅为研究TBI的分子机制提供了新的方向，而且为通过调控circRNA治疗TBI提供了新的可能性。
Circular RNAs (circRNAs) are involved in a variety of diseases. However, the roles of circRNAs in traumatic brain injury (TBI) remain unknown. In this study, circRNA microarray was used to profile the altered circRNAs in the rat hippocampus following TBI. A total of 192 circRNAs were observed to be differentially expressed (fold change [FC] >= 1.5 and p < 0.05) after TBI, including 98 upregulated and 94 downregulated. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that many messenger RNAs (mRNAs) transcribed from the host genes of altered circRNAs were implicated in brain damage and neural regeneration. CircRNA/microRNA (miRNA) interaction was predicted using Arraystar's homemade miRNA target prediction software based on TargetScan and miRanda. Thus, our studies have demonstrated altered circRNA expression pattern in the rat hippocampus after TBI, which may play important roles in post-TBI physiological and pathological processes. These findings may provide not only a new direction for studying the molecular mechanisms underlying TBI but also a new possibility for the treatment of TBI by modulating circRNAs.