Iodination of thyroid-stimulating hormone for receptor-binding studies with human thyroid membranes: effects of specific activity and method of iodination.
Iodination of thyroid-stimulating hormone for receptor-binding studies with human thyroid membranes: effects of specific activity and method of iodination.
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用于人类甲状腺膜受体结合研究的促甲状腺激素的碘化：比活性和碘化方法的影响。
DOI：
10.1677/joe.0.0840439
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
B. D. Thompson
中科院分区：
文献类型：
--
作者：
J. Kermode;B. D. Thompson
对用于人体甲状腺膜受体结合研究的促甲状腺激素 (TSH) 的 125I 标记的三种不同方法进行了比较：这些方法是氯胺-T、Bolton-Hunter 和乳过氧化物酶方法。事实证明，氯胺-T 方法不如其他两种方法。还比较了不同比活性 (0.7--9.4 Bq/pg) 的碘化：过高的比活性导致结合减少以及 TSH-受体相互作用的最佳 pH 值发生显着变化。如果 125I 标记的 TSH 的结合要代表天然激素的结合，则不应超过 2.5 Bq/pg 的比活性。在这些条件下，pH 7.5 最适合 TSH 与其受体的结合。通过受体吸附重新纯化标记的 TSH 也被证明是必要的。
Three different methods were compared for 125I-labelling of thyroid-stimulating hormone (TSH) for use in receptor-binding studies with human thyroid membranes: these were the chloramine-T, Bolton-Hunter and lactoperoxidase methods. Chloramine-T proved to be an inferior method to the other two. Iodinations to different specific activity (0.7--9.4 Bq/pg) were also compared: too high a specific activity led to reduced binding and a dramatic shift in the pH optimum for the TSH-receptor interaction. A specific acitivity of 2.5 Bq/pg should not be exceeded if binding of 125I-labelled TSH is to be representative of the binding of the natural hormone. Under these conditions, pH 7.5 was optimal for binding of TSH to its receptor. Repurification of the labelled TSH by receptor adsorption also proved to be essential.