Photoprotective Effects of Two Natural Products on Ultraviolet B-Induced Oxidative Stress and Apoptosis in SKH-1 Mouse Skin
Photoprotective Effects of Two Natural Products on Ultraviolet B-Induced Oxidative Stress and Apoptosis in SKH-1 Mouse Skin
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DOI：
10.1089/jmf.2010.0142
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发表时间：
2011-07-01
影响因子：
2.4
通讯作者：
Postescu, Ion Dan
中科院分区：
文献类型：
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作者：
Filip, Adriana;Daicoviciu, Doina;Postescu, Ion Dan
太阳紫外线辐射 (UV) 是人类非黑色素瘤皮肤癌的主要原因。使用天然产品进行光化学预防是治疗皮肤肿瘤的一种简单但非常有效的策略。我们在 SKH-1 无毛小鼠皮肤模型中研究了马蹄莲和红葡萄籽（Vitis vinifera L，勃艮第马品种 [BM]）提取物的体内光保护活性。将 50 只 8 周龄雌性 SKH-1 无毛小鼠随机分为 5 组（每组 10 只）：对照组、UVB 照射组、普通念珠菌加 UVB 照射组、BM 加 UVB 照射组、表没食子儿茶素没食子酸酯 (EGCG) 加 UVB 照射组。在单剂量 (240 mJ/cm(2)) UVB 照射前 30 分钟，将两种提取物的剂量为每 cm(2) 皮肤面积 4 mg/小鼠局部涂抹到小鼠身上。将溶解在磷酸盐缓冲盐水（pH 6.6；0.067 M）中的 EGCG 以 2 mg/小鼠/cm(2) 给药。照射后 24 小时测定皮肤匀浆中的谷胱甘肽过氧化物酶和过氧化氢酶活性、还原型谷胱甘肽 (GSH)、丙二醛、一氧化氮和半胱天冬酶 3 活性。单剂量的 UVB 会增加暴露皮肤中的谷胱甘肽水平和谷胱甘肽过氧化物酶活性。 C. vulgaris 和 BM 预处理显着降低 GSH 形成和谷胱甘肽过氧化物酶活性 (P < .001)，并抑制 UVB 诱导的脂质过氧化 (P < .0001) 和一氧化氮产生 (C. vulgaris: P < .06)。使用 C. vulgaris，尤其是 BM 提取物进行局部治疗 (P < .002) 显着降低 caspase 3 活性，表明细胞受到保护，免于凋亡。这些结果表明，普通念珠菌和BM提取物可能是降低紫外线诱发的皮肤癌风险的化学预防候选者。
Solar ultraviolet radiation (UV) is the major cause of nonmelanoma skin cancer in humans. Photo-chemoprevention with natural products represents a simple but very effective strategy for the management of cutaneous neoplasia. We studied the photoprotective activity of Calluna vulgaris and red grape seed (Vitis vinifera L, Burgund Mare variety [BM]) extracts in vivo in an SKH-1 hairless mice skin model. Fifty 8-week-old female SKH-1 hairless mice were randomly divided into 5 groups (n = 10 each): controls, UVB-irradiated, C. vulgaris plus UVB-irradiated, BM plus UVB-irradiated, and epigallocatechin gallate (EGCG) plus UVB-irradiated. A dose of 4 mg/mouse per cm(2) of skin area for both extracts was topically applied to the mice 30 minutes before a single-dose (240 mJ/cm(2)) UVB exposure. EGCG dissolved in phosphate-buffered saline (pH 6.6; 0.067 M) was administered at 2 mg/mouse per cm(2). Glutathione peroxidase and catalase activities, reduced glutathione (GSH), malondialdehyde, nitric oxide, and caspase 3 activity were determined in skin homogenates 24 hours after irradiation. A single dose of UVB increased GSH levels and glutathione peroxidase activity in the exposed skin. C. vulgaris and BM pretreatment significantly decreased GSH formation and glutathione peroxidase activity (P < .001) and inhibited UVB-induced lipid peroxidation (P < .0001) and nitric oxide production (C. vulgaris: P < .06). Topical treatments with C. vulgaris and particularly BM extracts (P < .002) significantly reduced caspase 3 activity, indicating that the cells were protected against apoptosis. These results suggest that C. vulgaris and BM extracts might be chemopreventive candidates for reducing UV-induced risk for skin cancer.