Genetically Engineered Liquid-Crystalline Viral Films for Directing Neural Cell Growth
Genetically Engineered Liquid-Crystalline Viral Films for Directing Neural Cell Growth
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DOI：
10.1021/la100226u
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发表时间：
2010-06-15
期刊：
影响因子：
3.9
通讯作者：
Lee, Seung-Wuk
中科院分区：
文献类型：
--
作者：
Chung, Woo-Jae;Merzlyak, Anna;Lee, Seung-Wuk
设计具有精确控制的结构组织的仿生基质，提供生物化学和物理线索来调节细胞行为，对于组织再生材料的开发至关重要。我们已经开发出由基因工程M13噬菌体（病毒）制成的新型液晶膜基质，其表现出控制和引导细胞行为的能力，用于组织再生应用。为了促进病毒和细胞之间的粘附，将2700个拷贝的M13主要外壳蛋白基因工程化以展示整合素结合肽（RGD）。所得到的纳米纤维状病毒显示的RGD基序与神经元细胞的生物相容性，并可以通过简单的剪切方法自组装形成长程有序的液晶基质。由此产生的排列结构能够决定细胞生长的方向。这些基于病毒的材料在体内再生靶组织的未来用途将为各种组织治疗提供巨大的机会。
Designing biomimetic matrices with precisely controlled structural organization that provides biochemical and physical cues to regulate cell behavior is critical for the development of tissue-regenerating materials. We have developed novel liquid-crystalline film matrices made from genetically engineered M13 bacteriophages (viruses) that exhibit the ability to control and guide cell behavior for tissue-regenerating applications. To facilitate adhesion between the viruses and cells, 2700 copies of the M 13 major coat protein were genetically engineered to display integrin-binding peptides (RGD). The resulting nanofiber-like viruses displaying RGD motifs were biocompatible with neuronal cells and could be self-assembled to form long-range-ordered liquid-crystalline matrices by a simple shearing method. The resulting aligned structures were able to dictate the direction of cell growth. Future use of these virus-based materials for regenerating target tissues in viva would provide great opportunities for various tissue therapies.