Modest environmental enrichment: Effect on a radial maze validation and well being of rats
Modest environmental enrichment: Effect on a radial maze validation and well being of rats
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DOI：
10.1016/j.brainres.2005.06.069
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发表时间：
2005-08-30
期刊：
影响因子：
2.9
通讯作者：
de Seze, R
中科院分区：
文献类型：
--
作者：
Brillaud, E;Morillion, D;de Seze, R
我们的8臂放射状迷宫测试被验证，以证明与毒蕈碱拮抗剂东莨菪碱氢溴酸盐（SHB，0.1毫克/公斤，腹腔注射）治疗的大鼠的记忆缺陷。为了提高生活质量，我们丰富了单圈养大鼠的环境。选择富集程序是为了增加动物的健康，而不会干扰很多行为测试的结果。它是温和的，由一个塑料管和玉米片组成。在8臂径向迷宫验证期间，比较了丰富环境（EE）和非丰富环境（NE）动物的表现。选择富集程序以增加动物的健康，而不干扰行为测试的结果。然后评估我们的富集条件对大鼠的一般行为、体重变化和十字迷宫焦虑测试结果的影响。结果显示，SHB治疗的动物在学习方面存在缺陷和延迟，以及一般的时间依赖性学习效应，验证了我们的测试。未观察到富集对阴性对照动物的影响。对于SHB处理的动物，丰富的学习任务期间的性能增加，并加重测试任务的缺陷。丰富的动物的探索行为似乎增加。发现EE动物的健康状况总体改善（体重稳定）。我们的结论是，我们的富集允许增加探索行为，而不是使用我们的方案的简单修改（限制为16次访问/试验）修改径向迷宫的敏感性。我们决定将这种丰富推广到我们所有的研究中，因为它很简单，而且获得了好处。(c)2005 Elsevier B. V.保留所有权利。
Our 8-arm radial maze test was validated to demonstrate memory deficits in rats treated with the muscarinic antagonist scopolamine hydro bromide (SHB, 0.1 mg/kg, i.p.). To improve quality of life, we enriched the environment of single housing rats. Enrichment procedures were chosen to increase the animals' well being without disturbing a lot the results of behavioural tests. It is modest, consisting of a plastic tube and corn chips. Enriched environment (EE) and Non-enriched Environment (NE) animals' performances were compared during the 8-arms radial maze validation. Enrichment procedures were chosen to increase the animals' well being without disturbing the results of behavioural tests. The impact of our enrichment conditions was then evaluated on the general behaviour of rats, weight evolution and results of a plus maze anxiety test. Results showed a deficit and a delay in learning for SHB-treated animals, and a general time-dependent learning effect, validating our test. No effect of enrichment on negative control animals was observed. For SHB-treated animals, enrichment increased performances during learning task and accentuated the deficits in test task. Exploratory behaviour of enriched animals seemed to be increased. A general amelioration of well being for EE animals was found (stable weight). We conclude that our enrichment allows increasing exploratory behaviour not modifying radial maze sensitivity using a simple modification of our protocol (limitation to 16 visits/trial). We decided to generalise this enrichment to all our studies, given its simplicity and obtained benefits. (c) 2005 Elsevier B.V. All rights reserved.