DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot in Arabidopsis.
DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot in Arabidopsis.
复制标题
DNA聚合酶ε与SUVH2/9相互作用抑制拟南芥减数分裂DSB热点相关基因的表达
DOI：
10.1073/pnas.2208441119
复制
发表时间：
2022-10-11
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
--
作者：
减数分裂重组是产生遗传多样化的单倍体配子所必需的，并且始于双链断裂（DSBs）的程序化形成，双链断裂通常发生在具有主动转录特征的可接近染色质中。然而，基因转录调控与DSB热点之间的关系尚不清楚。在这里，我们证明了最大的DNA聚合酶epsilon亚基POL2A将SU(VAR)3-9同源物SUVH2招募到减数分裂DSB位点，从而通过非经典RdDM途径共同抑制减数分裂DSB相关基因的转录。该研究为揭示植物减数分裂DSB位点基因转录被抑制的机制提供了新的见解，这在其他生物中可能是保守的。
Significance Meiotic recombination is essential for producing genetically diversified haploid gametes and initiates from the programmed formation of double-strand breaks (DSBs), which usually take place in accessible chromatin with features of active transcription in multiple species. However, the relationship between the regulation of gene transcription and DSB hotspots is not well understood. Here, we demonstrate that the largest DNA polymerase epsilon subunit POL2A recruits SU(VAR)3-9 homologs SUVH2 to the meiotic DSB sites, thereby jointly repressing the transcription of meiotic DSB-associated genes via a nonclassical RdDM pathway. This study provides new insights into how gene transcription is suppressed at meiotic DSB sites in plants, which could be conserved in other organisms.
登录
查看更多内容
影响因子：
30.8
作者：
Choi, Kyuha;Zhao, Xiaohui;Kelly, Krystyna A.;Venn, Oliver;Higgins, James D.;Yelina, Nataliya E.;Hardcastle, Thomas J.;Ziolkowski, Piotr A.;Copenhaver, Gregory P.;Franklin, F. Chris H.;McVean, Gil;Henderson, Ian R.
通讯作者：
Henderson, Ian R.
影响因子：
4.5
作者：
Choi K;Reinhard C;Serra H;Ziolkowski PA;Underwood CJ;Zhao X;Hardcastle TJ;Yelina NE;Griffin C;Jackson M;Mézard C;McVean G;Copenhaver GP;Henderson IR
通讯作者：
Henderson IR
影响因子：
14.9
作者：
Catoni M;Tsang JM;Greco AP;Zabet NR
通讯作者：
Zabet NR
影响因子：
14.9
作者：
Del Olmo I;López JA;Vázquez J;Raynaud C;Piñeiro M;Jarillo JA
通讯作者：
Jarillo JA
影响因子：
3.3
作者：
Han, Yonghua;Zhang, Tao;Jiang, Jiming
通讯作者：
Jiang, Jiming