Detection of Zn2+, Cd2+, Hg2+, and Pb2+ ions through label-free poly-L-glutamic acid.
Detection of Zn2+, Cd2+, Hg2+, and Pb2+ ions through label-free poly-L-glutamic acid.
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DOI：
10.1016/j.talanta.2018.06.012
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发表时间：
2018-10
期刊：
影响因子：
6.1
通讯作者：
Jingqiu Hu;Michael S. Elioff
中科院分区：
文献类型：
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作者：
Jingqiu Hu;Michael S. Elioff
水中重金属离子的检测对于环境可持续性和食品安全具有重要意义。目前的荧光传感器直接通过螯合或化学反应与金属离子相互作用。这些传感器的生产成本很高，而且通常一次只能检测一种离子。在这里，我们报告了一种荧光开启传感器，可以检测三个IIB族金属离子和Pb2+离子通过无标记的多肽在水中。在我们的传感器-多肽混合物中，Zn~（2+）、Cd~（2+）、Hg~（2+）和Pb~（2+）离子诱导聚-L-α-谷氨酸（PGA）的螺旋形成和链间聚集。基于吖啶鎓的传感器分子掺入多肽中，并在UV灯下强烈发射每个IIB族离子的特征颜色。通过调节多肽的大小或侧链羧基的长度，我们可以选择性地关闭或打开Hg 2+离子的传感器发射。
Detection of heavy metal ions in water is important for environmental sustainability and food safety. Current fluorescent sensors interact with metal ions directly through chelation or chemical reactions. Those sensors are expensive to produce and often can detect only one ion at a time. Here we report a fluorescent turn-on sensor that can detect three group IIB metal ions and Pb2+ions through label-free polypeptides in water. In our sensor-polypeptide mixture, Zn2+, Cd2+, Hg2+, and Pb2+ions induce helix formation and inter-chain aggregation in poly-L-α-glutamic acid (PGA). The acridinium-based sensor molecules incorporate into the polypeptides and emit strongly with characteristic color for each group IIB ion under UV lamp. By adjusting the size of polypeptides or the length of the side chain carboxyl groups, we can selectively turn off or turn on the sensor emission for Hg2+ions.