Gene conversion and end-joining-repair double-strand breaks in the Caenorhabditis elegans germline
Gene conversion and end-joining-repair double-strand breaks in the Caenorhabditis elegans germline
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DOI：
10.1534/genetics.108.089698
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发表时间：
2008-09-01
期刊：
影响因子：
3.3
通讯作者：
Bessereau, Jean-Louis
中科院分区：
文献类型：
--
作者：
Robert, Valrie J.;Davis, M. Wayne;Bessereau, Jean-Louis
切除秀丽隐杆线虫生殖系中的Mos1转座子在染色体中产生双链断裂，我们证明，最突出的是修复断裂的同源基因转换，但也很少，通过非同源末端连接。在一些情况下，基因转换事件通过交换来解决。令人惊讶的是，使用所有种系特异性启动子的转座酶表达可以诱导修复，提高了体细胞中转座酶表达的激活可以导致种系中Mos 1转座的可能性。
Excision of a Mos1 transposon in the germline of Caenorhabditis elegans generates a double-strand break In the chromosome.,We demonstrate that breaks are most prominently repaired by gene conversion from the homolog, but also rarely, by nonhomologous end-joining. In some cases, gene, conversion events are resolved by crossing over. Surprisingly, expression of the transposase using all intestine-specific promoter can induce repair, raising the possibility that activation of transposase expression in somatic cells can lead to transposition of Mos1 in the germline..