Effects of brefeldin A on the localization of Tobamovirus movement protein and cell-to-cell movement of the virus.
Effects of brefeldin A on the localization of Tobamovirus movement protein and cell-to-cell movement of the virus.
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DOI：
10.1016/j.virol.2006.11.008
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发表时间：
2007-04
期刊：
影响因子：
3.7
通讯作者：
Y. Tagami;Yuichiro Watanabe
中科院分区：
文献类型：
--
作者：
Y. Tagami;Yuichiro Watanabe
研究表明，与绿色荧光蛋白（MP：GFP）融合的烟草花叶病毒运动蛋白（MP）在感染过程中发生了亚细胞定位的变化。然而，MP的细胞内转运途径及其生物学意义仍然不清楚。治疗与布雷菲德菌素A（BFA），这破坏ER到高尔基体的运输，抑制MP的不规则形状和丝状结构的形成。在此条件下，MP仍然靶向叶细胞中的胞间连丝。此外，病毒细胞间的移动不受BFA处理的抑制。这些数据表明，靶向的病毒复制复合物（VRC）的胞间连丝介导的BFA不敏感的途径和ER到高尔基体的运输途径不参与病毒的细胞间运动。
It has been demonstrated that the subcellular location of Tobamovirus movement protein (MP) which was fused with green fluorescent protein (MP:GFP) changed during the infection process. However, the intracellular route through which MP is transported and its biological meaning are still obscure. Treatment with brefeldin A (BFA), which disrupts ER-to-Golgi transport, inhibited the formation of irregularly shaped and filamentous structures of MP. In this condition, MP was still targeted to plasmodesmata in leaf cells. Furthermore, the viral cell-to-cell movement was not inhibited by BFA treatment. These data indicated that the targeting of viral replication complexes (VRCs) to plasmodesmata is mediated by a BFA-insensitive pathway and that the ER-to-Golgi transport pathway is not involved in viral intercellular movement.