LEPROSY 1962-1992 .2. THE MICROBIOLOGY OF MYCOBACTERIUM-LEPRAE - PROGRESS IN THE LAST 30 YEARS
LEPROSY 1962-1992 .2. THE MICROBIOLOGY OF MYCOBACTERIUM-LEPRAE - PROGRESS IN THE LAST 30 YEARS
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DOI：
10.1016/0035-9203(93)90064-w
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发表时间：
1993-09-01
影响因子：
2.2
通讯作者：
COLSTON, MJ
中科院分区：
文献类型：
--
作者：
COLSTON, MJ
在过去的30年里，在微生物实验室研究麻风分枝杆菌的方式发生了巨大的变化。这种生物还没有在体外培养，但从在老鼠脚垫上的生长演示开始，到分子生物学和遗传技术的应用，我们现在能够绕过由于缺乏可培养性而产生的一些困难。这些研究为我们提供了对生物体基本生物学的新见解，并可能为临床实验室提供有价值的新工具。本文简要介绍了分子技术在细菌鉴定和耐药检测等领域的研究进展及应用前景。
Over the last 30 years, there have been dramatic changes in the way Mycobacterium leprae is studied in the microbiology laboratory. The organism still has not been grown in vitro but starting with demonstration of growth in the footpads of mice and culminating in the application of molecular biological and genetic techniques, we are now in a position to circumvent some of the difficulties arising from lack of cultivability. Such studies are providing us with new insights into the basic biology of the organism and are likely to provide new tools which will be of value in the clinical laboratory. In this article, I briefly outline the progress which has been made, and the potential applications of molecular techniques in such areas as bacterial identification and drug-resistance testing.