Interleukin-10 inhibits cytokine synthesis in monocytes stimulated by titanium particles: evidence of an anti-inflammatory regulatory pathway.
Interleukin-10 inhibits cytokine synthesis in monocytes stimulated by titanium particles: evidence of an anti-inflammatory regulatory pathway.
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Interleukin-10 抑制钛颗粒刺激的单核细胞中细胞因子的合成：抗炎调节途径的证据。
DOI：
10.1002/jor.1100160611
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
O'Keefe,RJ
中科院分区：
文献类型：
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作者：
Pollice,PF;Hsu,J;Hicks,DG;Bukata,S;Rosier,RN;Reynolds,PR;Puzas,JE;O'Keefe,RJ
研究抗炎介质白介素-10 作为钛颗粒激活的人外周血单核细胞中促炎细胞因子释放的潜在抑制剂。它以剂量依赖性方式抑制肿瘤坏死因子-α 和白细胞介素-6 的分泌，在 2 ng/ml 时观察到完全抑制。进行共培养实验以确定这种细胞因子作为炎症反应抑制剂是否具有重要的功能。当未刺激的淋巴细胞和单核细胞与钛刺激的单核细胞共培养时，它们显着抑制了白细胞介素-6和肿瘤坏死因子-α的分泌。添加白介素-10 中和抗体可以部分阻断这些共培养细胞的抑制作用。在用钛颗粒处理的单核细胞培养物以及用钛刺激单核细胞的条件培养基处理的新鲜单核细胞培养物中测量了白介素-10 水平。后面的实验证明了条件培养基处理的培养物中白细胞介素 10 分泌的显着刺激，这种效应与条件培养基中肿瘤坏死因子 α 的存在有关。在条件培养基处理的培养物中添加钛显着减少了白细胞介素-10的分泌，这表明反应最灵敏的细胞是暴露于活化单核细胞释放的试剂的未刺激单核细胞。总而言之，白介素-10 的表达和反应性表明抗炎细胞因子在调节磨损碎片的炎症反应中具有潜在作用。
The anti‐inflammatory mediator interleukin‐10 was investigated as a potential inhibitor of pro‐inflammatory cytokine release in human peripheral blood monocytes activated with titanium particles. It inhibited the secretion of both tumor necrosis factor‐alpha and interleukin‐6 in a dose‐dependent manner, with complete inhibition observed at 2 ng/ml. Co‐culture experiments were performed to determine whether this cytokine may have functional importance as an inhibitor of the inflammatory response. When unstimulated lymphocytes and monocytes were co‐cultured with titanium‐stimulated monocytes, they significantly suppressed the secretion of both interleukin‐6 and tumor necrosis factor‐alpha. The inhibitory effect of these co‐cultured cells could be partially blocked with the addition of an interleukin‐10 neutralizing antibody. Interleukin‐10 levels were measured in monocyte cultures treated with titanium particles as well as in fresh monocyte cultures treated with conditioned medium from titanium‐stimulated monocytes. The latter experiments demonstrated marked stimulation of interleukin‐10 secretion in conditioned medium‐treated cultures, an effect that was related to the presence of tumor necrosis factor‐alpha in the conditioned medium. The addition of titanium to conditioned medium‐treated cultures markedly reduced the secretion of interleukin‐10, suggesting that the most responsive cells are unstimulated monocytes exposed to agents released from activated monocytes. Altogether, the expression and responsiveness to interleukin‐10 suggest a potential role for anti‐inflammatory cytokines in regulation of the inflammatory response to wear debris.