Use of a proposed antimicrobial susceptibility testing method for Haemophilus parasuis
Use of a proposed antimicrobial susceptibility testing method for Haemophilus parasuis
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DOI：
10.1016/j.vetmic.2014.06.010
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发表时间：
2014-08-27
影响因子：
3.3
通讯作者：
Turni, Conny
中科院分区：
文献类型：
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作者：
Dayao, Denise Ann E.;Kienzle, Marco;Turni, Conny
本研究的目的是检查97个从澳大利亚猪中培养的阳伞嗜血杆菌的抗菌药物敏感性。由于目前尚无标准的H.对于副猪链球菌，最初使用临床和实验室标准化研究所推荐的参考菌株评价了使用补充培养基、用于纸片扩散的BA/SN和用于微量稀释技术的试验培养基肉汤（TMB）的方法。结果表明，BA/SN和TMB可作为H.副猪。然后使用所提出的微量稀释技术与97 H。阳伞分离物和9种抗菌剂。研究发现，澳大利亚分离株对氨苄青霉素（1%）、青霉素（2%）、红霉素（7%）、妥拉霉素（9%）、替米考星（22%）、四环素（31%）和甲氧苄啶-磺胺甲恶唑（40%）的最低抑菌浓度（MIC）升高。本研究描述了潜在的抗菌药物敏感性的方法，H。阳伞，并已检测到低百分比的澳大利亚H。阳伞分离物具有升高的抗菌MIC。皇冠版权所有（C）2014由爱思唯尔B. V.出版。保留所有权利。
The aim of this study was to examine the antimicrobial susceptibility of 97 Haemophilus parasols cultured from Australian pigs. As there is no existing standard antimicrobial susceptibility technique available for H. parasuis, methods utilising the supplemented media, BA/SN for disc diffusion and test medium broth (TMB) for a microdilution technique, were initially evaluated with the reference strains recommended by the Clinical and Laboratory Standards Institute. The results of the media evaluation suggested that BA/SN and TMB can be used as suitable media for susceptibility testing of H. parasuis. The proposed microdilution technique was then used with 97 H. parasols isolates and nine antimicrobial agents. The study found that Australian isolates showed elevated minimum inhibitory concentrations (MICs) for ampicillin (1%), penicillin (2%), erythromycin (7%), tulathromycin (9%), tilmicosin (22%), tetracycline (31%) and trimethoprim-sulfamethoxazole (40%). This study has described potential antimicrobial susceptibility methods for H. parasols and has detected a low percentage of Australian H. parasols isolates with elevated antimicrobial MICs. Crown Copyright (C) 2014 Published by Elsevier B.V. All rights reserved.