Transcriptome changes associated with instructed learning in the barn owl auditory localization pathway.
Transcriptome changes associated with instructed learning in the barn owl auditory localization pathway.
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转录组变化与仓鸮听觉定位通路中的指导学习相关。
DOI：
10.1002/dneu.20458
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发表时间：
2007
影响因子：
3
通讯作者：
DeBello,WilliamM
中科院分区：
文献类型：
--
作者：
Swofford,JanetA;DeBello,WilliamM
戴着棱镜眼镜饲养的猫头鹰学会了做出适应性的定向动作。这种指令学习依赖于中脑听觉空间图的重新校准，这反过来又涉及新突触的形成。在这里，我们调查这些过程是否与差异基因表达，使用longSAGE。新羽翼丰满的猫头鹰饲养了8-36天的棱镜或控制镜头，在这个时候的学习程度进行了量化的电生理映射。从下丘（IC），突触可塑性的主要部位，和视顶盖（OT），这提供了一个指导性的信号，控制可塑性的方向和程度的转录组配置文件。在超过35，000个独特标签中，在IC中鉴定出22个差异表达序列标签，在OT中鉴定出36个差异表达序列标签。其中，只有四个在这两种结构中受到监管。这些结果表明，两个很大程度上独立的基因簇的调节与突触重塑（IC）和指导信号的产生（OT）。真实的时间PCR数据证实了两种转录物的变化，泛素/聚泛素和酪氨酸3-单加氧酶/色氨酸5-单加氧酶激活蛋白，θ亚基（YWHAQ;也称为14 - 3 - 3蛋白）。泛素在IC中下调，与蛋白质降解途径作为突触发生抑制性约束的模型一致。YWHAQ在OT中上调，表明在指导性信息的合成或传递中发挥作用。总的来说，我们的结果提供了一条解开分子级联的途径，这些分子级联将自然主义经验与突触重塑以及最终与习得行为的表达联系起来。© 2007威利期刊公司开发神经生物学，2007年。
Owls reared wearing prismatic spectacles learn to make adaptive orienting movements. This instructed learning depends on re‐calibration of the midbrain auditory space map, which in turn involves the formation of new synapses. Here we investigated whether these processes are associated with differential gene expression, using longSAGE. Newly fledged owls were reared for 8–36 days with prism or control lenses at which time the extent of learning was quantified by electrophysiological mapping. Transciptome profiles were obtained from the inferior colliculus (IC), the major site of synaptic plasticity, and the optic tectum (OT), which provides an instructive signal that controls the direction and extent of plasticity. Twenty‐two differentially expressed sequence tags were identified in IC and 36 in OT, out of more than 35,000 unique tags. Of these, only four were regulated in both structures. These results indicate that regulation of two largely independent gene clusters is associated with synaptic remodeling (in IC) and generation of the instructive signal (in OT). Real‐time PCR data confirmed the changes for two transcripts, ubiquitin/polyubiquitin and tyrosine 3‐monooxgenase/tryotophan 5‐monooxygenase activation protein, theta subunit (YWHAQ; also referred to as 14‐3‐3 protein). Ubiquitin was downregulated in IC, consistent with a model in which protein degradation pathways act as an inhibitory constraint on synaptogenesis. YWHAQ was up‐regulated in OT, indicating a role in the synthesis or delivery of instructive information. In total, our results provide a path towards unraveling molecular cascades that link naturalistic experience with synaptic remodeling and, ultimately, with the expression of learned behavior. © 2007 Wiley Periodicals, Inc. Develop Neurobiol, 2007.