Duck Tembusu Virus Exhibits Pathogenicity to Kunming Mice by Intracerebral Inoculation.
Duck Tembusu Virus Exhibits Pathogenicity to Kunming Mice by Intracerebral Inoculation.
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鸭天宝苏病毒脑内接种对昆明小鼠表现出致病性
DOI：
10.3389/fmicb.2016.00190
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发表时间：
2016
影响因子：
5.2
通讯作者：
Diao Y
中科院分区：
文献类型：
--
作者：
Ti J;Zhang M;Li Z;Li X;Diao Y
本研究以昆明种小鼠为动物模型，研究TMUV的致病性。将TMUV SDSG株50 μL尿囊液（104.8ELD_（50）/0.2ml）分别经脑内（i.c.）皮下（s.c.）和鼻内（i.n.）航线对照组（n= 15只小鼠）接种50 μL无菌磷酸盐缓冲盐水。检查并记录临床体征、大体和显微镜病变、不同组织中的病毒载量和血清抗体滴度。昆明种小鼠脑内感染后表现出典型的临床症状，包括严重的后肢瘫痪、体重减轻和死亡。仅死亡小鼠出现严重肠粘膜水肿。在连续人道处死的小鼠中未观察到肉眼病变。然而，在脑，脾，肝，肾，肺的显微镜下病变是非常典型的，包括不同程度的病毒性脑炎，淋巴细胞耗竭，肝细胞坏死和肾炎等。不同组织中的病毒载量通过SYBR绿色I实时PCR检测。病毒载量在脑、肝和脾中最先检测到，并维持较长时间，表明这些器官可能是TMUV的靶器官。病毒载量水平与不同组织中临床体征和显微镜病变的严重程度一致。中和抗体在8dpi开始血清转化。其他各组均未观察到临床症状、镜下病变、病毒载量和血清中和抗体。综上所述，TMUV通过i.c.可引起昆明小鼠全身感染和死亡，为进一步研究TMUV在公共卫生中的意义提供了一定的实验依据。
In this study, Kunming mice were used as the animal models to study the pathogenicity of TMUV. Three groups of 3-week-old female Kunming mice (n= 15 mice per group) were infected with the SDSG strain of TMUV in 50 μL allantoic fluid (104.8ELD50/0.2 ml) respectively by the intracerebral (i.c.), subcutaneous (s.c.) and intranasal (i.n.) routes. The control group (n= 15 mice) was inoculated with 50 μL sterile phosphate-buffered saline. Clinical signs, gross, and microscopic lesions, viral loads in different tissues, and serum antibody titers were examined and recorded. Kunming mice infected intracerebrally showed typical clinical symptoms, including severe hindlimb paralysis, weight loss and death. Only dead mice presented severe intestinal mucosal edema. No gross lesions were observed in mice sequentially euthanized. However, microscopic lesions in the brain, spleen, liver, kidney, and lung were very typical including varying degrees of viral encephalitis, lymphocytes depletion, liver cell necrosis and nephritis, etc. Viral loads in different tissues were detected by the SYBR Green I real-time PCR assay. Viral loads in the brain, liver, and spleen were first detected and maintained a longer time, which indicated that these organs may be the target organs of TMUV. The level of viral loads was consistent with the severity of clinical signs and microscopic lesions in different tissues. The neutralizing antibody began to seroconvert at 8 dpi. Clinical signs, microscopic lesions, viral loads and serum neutralizing antibodies weren’t observed in other groups. In summary, TMUV can cause systemic infections and death in Kunming mice by i.c., which provides some experimental basis for further study of the significance of TMUV in public health.