Evaluation of an infrared laser-Doppler blood flowmeter.
Evaluation of an infrared laser-Doppler blood flowmeter.
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红外激光多普勒血流量计的评估。
DOI：
10.1152/ajpgi.1987.252.6.g832
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Maxwell,LC
中科院分区：
文献类型：
--
作者：
Shepherd,AP;Riedel,GL;Kiel,JW;Haumschild,DJ;Maxwell,LC
目前市场上有几种激光多普勒血流计;然而，只有一种使用红外激光二极管（Laserflo，TSI，圣保罗，MN）。由于这一点和其他独特的功能，如其基于微处理器的信号分析仪，我们评估了该设备的能力，测量组织灌注。最初，我们确定激光照射是否直接影响微血管。仓鼠提睾肌活体显微镜观察表明，既不氦氖也不红外激光影响的直径的小动脉，血管活性剂的反应。为了测试流量计的线性度和可重复性，我们使用旋转盘来模拟光散射流动介质。“流动”信号与盘的旋转速度高度相关（r = 0.99），在流动探针之间是一致的，并且显示出高度的再现性。第二个模型由通过比色皿泵送的微球悬浮液组成。激光多普勒流速仪（LDV）的流量信号与泵的输出呈线性关系。在灌注液中有红细胞的情况下，我们检查了血液氧合对流量计性能的影响。LDV血流信号不受血氧变化的影响。我们在离体灌注大鼠肝脏和离体犬胃瓣中评价了体内线性。我们观察到的线性关系，总流量和激光多普勒血流测量的表面上的肝脏（r = 0.98）和胃粘膜（r = 0.98），但总的和局部LDV流量之间的关系的斜率显示相当大的变异性没有注意到在体外研究。（250字处删节）
Several laser-Doppler blood flowmeters are now commercially available; however, only one utilizes an infrared laser diode (Laserflo, TSI, St. Paul, MN). Because of this and other unique features such as its microprocessor-based signal analyzer, we evaluated this device's ability to measure tissue perfusion. Initially, we determined whether laser illumination directly affected the microvasculature. Intravital microscopic observations in the hamster cremaster muscle indicated that neither He-Ne nor infrared laser light affected the diameters of arterioles that were responsive to vasoactive agents. To test the flowmeter for linearity and repeatability, we used a rotating disk to simulate a light-scattering, flowing medium. The "flow" signal was highly correlated (r = 0.99) with the rotational velocity of the disk, was consistent among flow probes, and showed a high degree of reproducibility. The second model consisted of microsphere suspensions pumped through cuvettes. The laser-Doppler velocimeter (LDV) flow signal was linear with respect to pump output. With red blood cells in the perfusate, we examined the effects of blood oxygenation on the flowmeter's performance. The LDV flow signal was unaffected by changes in blood oxygenation. We evaluated linearity in vivo in isolated, perfused rat livers and in isolated canine gastric flaps. We observed linear relationships between total flow and laser-Doppler flow measured on the surface of the liver (r = 0.98) and in the gastric mucosa (r = 0.98), but the slopes of the relationships between total and local LDV flow showed considerable variability not noted in the in vitro studies.(ABSTRACT TRUNCATED AT 250 WORDS)