Structural organization of the perimysium in bovine skeletal muscle: Junctional plates and associated intracellular subdomains
Structural organization of the perimysium in bovine skeletal muscle: Junctional plates and associated intracellular subdomains
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DOI：
10.1016/j.jsb.2006.01.002
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发表时间：
2006-05-01
影响因子：
3
通讯作者：
Delage, JP
中科院分区：
文献类型：
--
作者：
Passerieux, E;Rossignol, R;Delage, JP
我们分析了牛桡侧腕屈肌肌束膜胶原网络的结构特征，使用各种样品制备方法和显微镜技术。我们首先通过扫描电子显微镜观察到肌束膜形成了规则的胶原纤维网络，具有三个层次，包括（i）松散的大交织纤维网格，（ii）许多胶原丛在（iii）特定结构的水平上连接在一起，我们称之为肌束膜连接板。其次，我们使用透射电子显微镜、免疫组织化学和连续切片的三维重建更仔细地观察了每个平板下的细胞内组织。我们观察到肌细胞核聚集成簇排列，周围有高密度的肌膜下线粒体和毛细血管分支。第三，我们分析了这些肌束膜连接板，肌膜下线粒体和肌核簇沿着肌纤维的分布，使用这些结构之间的距离的统计分析。这揭示了一个全球性的共定位和存在的粘附域之间的肌内膜和肌束膜。两者合计，我们的观察给出了一个更好的描述肌束膜组织在骨骼肌，并提供证据表明，肌束膜连接板与相关的细胞内子域可能参与肌收缩力的横向传输以及mechanosensing。(c)2006年爱思唯尔公司All rights reserved.
We analyzed the structural features of the perimysium collagen network in bovine Flexor carpi radialis muscle using various sample preparation methods and microscopy techniques. We first observed by scanning electron microscopy that perimysium formed a regular network of collagen fibers with three hierarchical levels including (i) a loose lattice of large interwoven fibers ramified ill (ii) numerous collagen plexi attaching together adjacent myofibers at the level of (iii) specific structures that we call perimysial junctional plates. Second, we looked more closely at the intracellular organization underneath each plate using transmission electron microscopy, immunohistochemistry, and a three-dimensional reconstruction from serial sections. We observed the accumulation of myonuclei arranged in clusters surrounded by a high density of subsarcolemmal mitochondria and the proximity of capillary branches. Third, we analyzed the distribution of these perimysial junctional plates, subsarcolemmal mitochondria, and myonuclei clusters along the myofibers using a statistical analysis of the distances between these structures. This revealed a global colocalization and the existence of adhesion domains between endomysium and perimysium. Taken together, our observations give a better description of the perimysium organization ill skeletal muscle, and provide evidence that perimysial junctional plates with associated intracellular subdomains may participate ill the lateral transmission of contractile forces as well as mechanosensing. (c) 2006 Elsevier Inc. All rights reserved.