Multiplex Immunosensor Arrays for Electrochemical Detection of Cancer Biomarker Proteins.
Multiplex Immunosensor Arrays for Electrochemical Detection of Cancer Biomarker Proteins.
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DOI：
10.1002/elan.201600183
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发表时间：
2016-11
期刊：
影响因子：
3
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Munge BS;Stracensky T;Gamez K;DiBiase D;Rusling JF
蛋白质生物标记物的测量面板为癌症早期检测、疾病监测和患者对治疗的反应提供了一种新的个性化方法。多重电化学方法具有独特的优势，可以提供更快、更灵敏的护理点(POC)设备来检测用于临床诊断的蛋白质生物标记物。基于纳米材料的电化学方法提供了早期癌症检测所需的灵敏度。本文综述了多重电化学免疫传感器在癌症诊断和疾病监测中的最新进展。讨论了不同的电化学方法，包括基于酶的免疫阵列、基于纳米颗粒的免疫阵列和电化学发光方法。这些方法中的许多已经被集成到微流控系统中，但在一个小的单一血清样本中测量2-4个以上的蛋白质标记物仍然是一个挑战。对于PoC应用，需要一种简单、低成本的方法。多重微流控免疫分析的主要挑战是试剂添加和洗涤步骤，这需要创造性的工程解决方案。还对三维打印微流控和纸基微流控器件进行了探索。
Measuring panels of protein biomarkers offer a new personalized approach to early cancer detection, disease monitoring and patients’ response to therapy. Multiplex electrochemical methods are uniquely positioned to provide faster, more sensitive, point of care (POC) devices to detect protein biomarkers for clinical diagnosis. Nanomaterials-based electrochemical methods offer sensitivity needed for early cancer detection. This review discusses recent advances in multiplex electrochemical immunosensors for cancer diagnostics and disease monitoring. Different electrochemical strategies including enzyme-based immunoarrays, nanoparticle-based immunoarrays and electrochemiluminescence methods are discussed. Many of these methods have been integrated into microfluidic systems, but measurement of more than 2–4 protein markers in a small single serum sample is still a challenge. For POC applications, a simple, low cost method is required. Major challenges in multiplexed microfluidic immunoassays are reagent additions and washing steps that require creative engineering solutions. 3-D printed microfluidics and paper-based microfluidic devices are also explored.