The endothelial glycocalyx in syndecan-1 deficient mice.
The endothelial glycocalyx in syndecan-1 deficient mice.
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DOI：
10.1016/j.mvr.2013.02.001
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发表时间：
2013-05
影响因子：
3.1
通讯作者：
Damiano, Edward R.
中科院分区：
文献类型：
--
作者：
Savery, Michele D.;Jiang, John X.;Park, Pyong Woo;Damiano, Edward R.
The existence of a hydrodynamically relevant endothelial glycocalyx has been established in capillaries, venules, and arterioles in vivo. The glycocalyx is thought to consist primarily of membrane-bound proteoglycans with glycosaminoglycan side-chains, membrane-bound glypicans, and adsorbed plasma proteins. The proteoglycans found on the luminal surface of endothelial cells are syndecans-1, -2, and -4, and glypican-1. The extent to which any of these proteins might serve to anchor the glycocalyx to the endothelium has not yet been determined. To test whether syndecan-1, in particular, is an essential anchoring protein, we performed experiments to determine the hydrodynamically relevant glycocalyx thickness in syndecan-1 deficient (Sdc1−/−) mice. Micro-particle image velocimetry data were collected using a previously described method. Microviscometric analysis of these data consistently revealed the existence of a hydrodynamically relevant endothelial glycocalyx in Sdc1−/− mice in vivo. The mean glycocalyx thickness found in Sdc1−/− mice was 0.45±0.10 μm (N=15), as compared with 0.54±0.12 μm (N=11) in wild-type (WT) mice (p=0.03). The slightly thinner glycocalyx observed in Sdc1−/− mice relative to WT mice may be due to the absence of syndecan-1. These findings show that healthy Sdc1−/− mice are able to synthesize and maintain a hydrodynamically relevant glycocalyx, which indicates that syndecan-1 is not an essential anchoring protein for the glycocalyx in Sdc1−/− mice. This may also be the case for WT mice; however, Sdc1−/− mice might adapt to the lack of syndecan-1 by increasing the expression of other proteoglycans. In any case, syndecan-1 does not appear to be a prerequisite for the existence of an endothelial glycocalyx.
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影响因子：
3.4
作者：
Damiano, ER;Stace, TM
通讯作者：
Stace, TM
影响因子：
--
作者：
DESJARDINS, C;DULING, BR
通讯作者：
DULING, BR
DOI：
10.1073/pnas.0402937101
发表时间：
2004-07-06
影响因子：
11.1
作者：
Long, DS;Smith, ML;Damiano, ER
通讯作者：
Damiano, ER
影响因子：
3.1
作者：
GRETZ, JE;DULING, BR
通讯作者：
DULING, BR
影响因子：
20.1
作者：
Chappell, Daniel;Jacob, Matthias;Becker, Bernhard F.
通讯作者：
Becker, Bernhard F.