Xray Irradiation Promotes Nerve Growth Factor-induced Neurite Extension in PC12 Cells
Xray Irradiation Promotes Nerve Growth Factor-induced Neurite Extension in PC12 Cells
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DOI：
10.3769/radioisotopes.65.137
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发表时间：
2016-03-01
期刊：
影响因子：
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通讯作者：
Suzuki, Takahiko
中科院分区：
文献类型：
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作者：
Katoh, Shinsuke;Kobayashi, Junya;Suzuki, Takahiko
神经生长因子（NGF）诱导PC12细胞的神经突延伸，这是一种众所周知的神经元分化模型。先前的报道表明，辐射激活细胞外信号调节激酶1/2 (ERK)，这是NGF信号传导的主流途径。这表明x射线照射可能会增强神经突的延伸。因此，我们在PC12细胞的x射线照射下观察到ngf诱导的神经突延伸。在NGF治疗开始时，前5分钟同时进行x射线照射。5天后，500 mGy的辐射促进了NGF诱导的神经突延伸，并增加了ERK的磷酸化，但没有增加NGF受体的磷酸化。照射也增强了PC12细胞中刺激生长信号表皮生长因子（EGF）受体的磷酸化。有趣的是，EGF受体酪氨酸激酶抑制剂AG1478抑制了辐射诱导的神经突延伸。这些结果表明，辐射激活EGF受体酪氨酸激酶，并通过NGF和EGF信号之间的串扰促进NGF诱导的神经突延伸。
Nerve growth factor (NGF) induces neurite extension in PC12 cells, a well-known model of neuronal differentiation. Previous reports have shown that irradiation activates the extracellular signal-regulated kinase 1/2 (ERK), which is a mainstream pathway for NGF signaling. This suggests that the neurite extension may possibly be enhanced by Xray irradiation. Therefore, we observed NGF-induced neurite extension with Xray irradiation in PC12 cells. At the start of the NGF treatment, Xray irradiation was performed simultaneously for the first 5 min. After 5 days, the irradiation at 500 mGy promoted NGF-induced neurite extension, and increased phosphorylation of ERK, but not of the NGF receptor. The phosphorylation of epidermal growth factor (EGF) receptor, a stimulatory growth signal in PC12 cells, was also enhanced by irradiation. Interestingly, AG1478, an inhibitor of EGF receptor tyrosine kinase, inhibited the irradiation-induced promotion of neurite extension. These results suggest that irradiation activate EGF receptor tyrosine kinase and promote NGF-induced neurite extension via the cross talk between NGF and EGF signaling.