Preparation and physicochemical properties of an injectable alginate-based hydrogel by the regulated release of divalent ions via the hydrolysis of d-glucono-delta-lactone
Preparation and physicochemical properties of an injectable alginate-based hydrogel by the regulated release of divalent ions via the hydrolysis of d-glucono-delta-lactone
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通过 d-葡萄糖酸-δ-内酯的水解调节二价离子释放的可注射海藻酸盐水凝胶的制备和理化性质
DOI：
10.1177/0885328219886185
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发表时间：
2020
影响因子：
2.9
通讯作者：
Yang Xianjin
中科院分区：
文献类型：
--
作者：
Sun Xiaojun;Li Zhaoyang;Cui Zhenduo;Wu Shuilin;Zhu Shengli;Liang Yanqin;Yang Xianjin
在这项研究中，可注射的多巴胺改性藻酸盐水凝胶与纳米羟基磷灰石或锶羟基磷灰石相结合，通过 D-葡萄糖酸-δ-内酯 (GDL) 水解过程中原位缓慢释放二价离子进行交联。交联机制涉及藻酸盐与二价离子的螯合以及藻酸盐和儿茶酚基团之间的氢键相互作用。由于0.5%（w/v）的GDL浓度调节了二价离子的释放，从而增强了螯合效应，因此研究人员将无机纳米颗粒均匀分布到水凝胶体系中，以改善机械性能并稳定水凝胶网络。此外，还研究了多巴胺对机械性能改善的影响。结果表明，在10%~50%应变值范围内，该可注射水凝胶的抗压强度比未添加GDL的水凝胶提高了4.6倍，与未改性的海藻酸盐水凝胶相比提高了4.0倍。与锶羟基磷灰石结合的水凝胶表现出更加致密、合适的孔径，具有三维网络结构，粒径为50-100μm；该值范围被认为是骨组织生长的最佳值。此外，通过调整实验变量，可以将凝胶时间从几分钟调整到几秒。我们的研究为提高骨组织工程材料的力学性能提供了一种潜在的方法。 (HNMR)-H-1、FTIR 和紫外-可见光谱测量； X射线衍射和扫描电镜；并进行了pH、离子释放和机械测试。
In this study, an injectable dopamine-modified alginate hydrogel combined with nano-hydroxyapatite or strontium hydroxyapatite was cross-linked via the in situ slow release of divalent ions from the hydrolysis of D-glucono-delta-lactone (GDL). The cross-linking mechanism involved the chelation of an alginate with divalent ions and the hydrogen-bonding interaction between alginate and catechol groups. As the GDL concentration at 0.5% (w/v) regulated the release of the divalent ions, which enhanced the chelation effect, it was investigated, and inorganic nanoparticles were homogeneously distributed into the hydrogel system to improve the mechanical properties and stabilize the hydrogel network. Furthermore, the effects of dopamine on the improvement of the mechanical properties were investigated. Results demonstrated that the compressive strength of this injectable hydrogel is enhanced by 4.6 times compared with that of the hydrogel without the addition of GDL, and the compressive strength is enhanced by 4.0 times compared with that of the unmodified alginate hydrogel at strain values ranging from 10% to 50%. The hydrogels combined with strontium hydroxyapatite exhibited a more compact, suitable pore size, with a three-dimensional network structure, and the particle size was 50-100 mu m; this range of values is considered as optimal for the growth of bone tissues. In addition, the gelation time can be adjusted from a few minutes to a few seconds by adjusting experiment variables. Our study provides a potential method for improving the mechanical properties of bone tissue engineering materials. (HNMR)-H-1, FTIR, and UV-vis spectroscopy measurements; XRD and SEM; and pH, ion release, and mechanical tests were carried out.