Natural killer T cells in families of patients with systemic lupus erythematosus - Their possible role in regulation of IgG production
Natural killer T cells in families of patients with systemic lupus erythematosus - Their possible role in regulation of IgG production
复制标题
DOI：
10.1002/art.22326
复制
发表时间：
2007-01-01
影响因子：
--
通讯作者：
Salaman, Myer R.
中科院分区：
文献类型：
--
作者：
Green, Matthew R. J.;Kennell, Amy S. M.;Salaman, Myer R.
Objective.目的探讨系统性红斑狼疮（SLE）患者及其亲属中自然杀伤T（NKT）细胞频率与IgG水平升高之间是否存在联系。血液样本来自SLE患者及其一级亲属、类风湿性关节炎（RA）患者和健康对照组。NKT细胞（定义为CD56+ T细胞）的频率表示为总血液淋巴细胞的百分比。检测血浆总IgG、IgM和抗双链DNA（dsDNA）IgG抗体水平。SLE患者的NKT细胞频率低于对照组。在SLE患者或RA患者的亲属中未观察到这种降低。在SLE患者及其亲属中观察到高水平的IgG，而在这些相同的组中观察到低水平的IgM。在SLE患者的亲属中，观察到NKT细胞的频率与IgG水平之间呈负相关。此外，SLE患者及其亲属IgG水平升高和患者IgG抗dsDNA水平升高与NKT细胞频率降低相关。这些结果表明，NKT细胞在调节IgG产生中具有重要作用，尽管具有不变T细胞受体的NKT细胞可能不一定参与其中。SLE背景下的NKT细胞可能缺乏来自NK或其他细胞的细胞因子刺激，这些细胞因子刺激是对IgG产生施加控制所需的。提高NKT细胞活性可能为SLE的治疗提供新的依据。
Objective. To determine whether there is a link between the frequency of natural killer T (NKT) cells and high levels of IgG in patients with systemic lupus erythematosus (SLE) and their relatives.Methods. Blood samples were obtained from patients with SLE, their first-degree relatives, patients with rheumatoid arthritis (RA), and healthy control subjects. The frequency of NKT cells (defined as CD56+ T cells) was expressed as a percentage of total blood lymphocytes. Plasma levels of total IgG and IgM, and IgG antibodies to double-stranded DNA (dsDNA) were determined.Results. The frequency of NKT cells was lower in patients with SLE than in control subjects. No such decrease was observed in the relatives of patients with SLE or in patients with RA. High levels of IgG were observed in both patients with SLE and their relatives, while low levels of IgM were observed in these same groups. In relatives of patients with SLE, an inverse correlation between the frequency of NKT cells and IgG levels was observed. Moreover, raised levels of IgG in patients with SLE and their relatives and high levels of IgG anti-dsDNA in patients were associated with low frequencies of NKT cells.Conclusion. These results suggest that NKT cells have an important role in the regulation of IgG production, although NKT cells with invariant T cell receptors may not necessarily be involved. NKT cells in the setting of SLE could lack the cytokine stimulus from NK or other cells that is needed to exert control on IgG production. Enhancement of NKT cell activity may provide a novel basis for therapy in SLE.