Pi64, Encoding a Novel CC-NBS-LRR Protein, Confers Resistance to Leaf and Neck Blast in Rice
Pi64, Encoding a Novel CC-NBS-LRR Protein, Confers Resistance to Leaf and Neck Blast in Rice
复制标题
DOI：
10.1094/mpmi-11-14-0367-r
复制
发表时间：
2015-05-01
影响因子：
3.5
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
Ma, Jian;Lei, Cailin;Wan, Jianmin
由稻瘟病引起的稻瘟病对全球水稻生产构成重大威胁。利用宿主抗性（R）基因被认为是控制稻瘟病最有效、最经济的手段。在这里，我们发现粳稻地方品种阳毛谷（YMG）比之前报道的其他广谱抗性（BSR）品种表现出更广泛的抗稻瘟病菌谱。遗传分析表明，YMG 至少包含三个主要 R 基因。一种基因 Pi64 对籼稻来源的分离株 CH43 和其他几个分离株表现出抗性，被定位到 YMG 1 号染色体上的 43 kb 间隔。编码核苷酸结合位点和富含亮氨酸的重复蛋白的两个开放阅读框（NBS-1和NBS-2）被列为Pi64的候选基因。将含有每个候选基因的构建体转化到三个易感粳稻品种中。只有带有 NBS-2 的转化体才具有对叶瘟和颈瘟的抗性，验证了 NBS-2 代表功能性 Pi64 基因的观点。 Pi64 在所有发育阶段和所有检查的组织中均持续表达。 Pi64 蛋白定位于细胞质和细胞核中。此外，通过基因转化和标记辅助选择将 Pi64 渗入易感品种，赋予籼稻来源的分离株高水平、广谱的叶瘟和颈瘟抗性，证明了其在育种 BSR 水稻品种中的潜在用途。
Rice blast caused by Magnaporthe oryzae poses a major threat to rice production worldwide. The utilization of host resistance (R) genes is considered to be the most effective and economic means to control rice blast. Here, we show that the japonica landrace Yangmaogu (YMG) displays a broader spectrum of resistance to blast isolates than other previously reported broad-spectrum resistant (BSR) cultivars. Genetic analysis suggested that YMG contains at least three major R genes. One gene, Pi64, which exhibits resistance to indica-sourced isolate CH43 and several other isolates, was mapped to a 43-kb interval on chromosome 1 of YMG. Two open reading frames (NBS-1 and NBS-2) encoding nucleotide-binding site and leucine-rich repeat proteins were short-listed as candidate genes for Pi64. Constructs containing each candidate gene were transformed into three susceptible japonica cultivars. Only transformants with NBS-2 conferred resistance to leaf and neck blast, validating the idea that NBS-2 represents the functional Pi64 gene. Pi64 is constitutively expressed at all development stages and in all tissues examined. Pi64 protein is localized in both the cytoplasm and nucleus. Furthermore, introgression of Pi64 into susceptible cultivars via gene transformation and marker-assisted selection conferred high-level and broad-spectrum leaf and neck blast resistance to indica-sourced isolates, demonstrating its potential utility in breeding BSR rice cultivars.