Human T-cell leukemia virus type 1 Tax protein induces the expression of lymphocyte chemoattractant SDF-1/PBSF
Human T-cell leukemia virus type 1 Tax protein induces the expression of lymphocyte chemoattractant SDF-1/PBSF
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DOI：
10.1006/viro.1997.8968
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发表时间：
1998-02-15
期刊：
影响因子：
3.7
通讯作者：
Fujii, M
中科院分区：
文献类型：
--
作者：
Arai, M;Ohashi, T;Fujii, M
我们研究了淋巴细胞浸润到感染人类 T 细胞白血病病毒 1 型 (HTLV-1) 的组织中的机制。细胞因子 SDF-1/PBSF 是一种高效的淋巴细胞趋化剂。逆转录PCR分析表明，在各种人类T细胞系中，感染HTLV-1的细胞选择性表达SDF-1基因。病毒蛋白Tax在人T细胞系中的表达诱导了SDF-1基因的表达，表明SDF-1在病毒感染的细胞系中的组成型表达至少部分是由Tax介导的。 HTLV-1 感染的 T 细胞系也表达 CXCR-4，SDF-1 的一种受体。此外，趋化性测定表明，HTLV-1 感染的细胞系向合成 SDF-1 迁移。因此，HTLV-1感染的细胞本身就是SDF-1的应答者。我们的结果表明，Tax 诱导的 SDF-1 可能会改变体内 HTLV-1 感染细胞的分布；因此，它可能有助于它们浸润到 HTLV-1 相关炎症性疾病的受影响组织中。 (C) 1998 年学术出版社。
We investigated the mechanism of lymphocyte infiltration into tissues infected with human T-cell leukemia virus type 1 (HTLV-1). The cytokine SDF-1/PBSF is a highly efficient chemoattractant for lymphocytes. Reverse transcription-PCR analysis showed that among various human T-cell lines, those infected with HTLV-1 selectively expressed the SDF-1 gene. Expression of the viral protein Tax in a human T-cell line induced the expression of the SDF-1 gene, indicating that the constitutive expression of SDF-1 in virus-infected cell lines is at least in part mediated by Tax. HTLV-1-infected T-cell lines also expressed CXCR-4, a receptor for SDF-1. Moreover, chemotaxis assay showed that a HTLV-1-infected cell line migrated toward synthetic SDF-1. Thus, HTLV-1-infected cells are themselves responders for SDF-1. Our results suggest that SDF-1 induced by Tax may alter the distribution of HTLV-1-infected cells in vivo; hence it may contribute to their infiltration into affected tissues in HTLV-1-associated inflammatory diseases. (C) 1998 Academic Press.