Impact of 4HPR on the expression of E-Cad in human bladder transitional epithelial cancer cells T24
Impact of 4HPR on the expression of E-Cad in human bladder transitional epithelial cancer cells T24
复制标题
4HPR对人膀胱移行上皮癌细胞T24中E-Cad表达的影响
DOI：
10.1007/s11596-012-0042-6
复制
发表时间：
2012
影响因子：
--
通讯作者：
Liu, Tongzu
中科院分区：
文献类型：
--
作者：
Wang, Eyou;Li, Jun;Yang, Guohua;Zhong, Shan;Liu, Tongzu
以往的研究表明，E-Cad在大多数浸润性癌细胞中的表达水平降低或呈阴性。本研究探讨4HPR是否通过调节E-Cad的表达来抑制膀胱癌细胞的侵袭。培养浸润性膀胱癌T24细胞，给予适当剂量的药物治疗。用四甲基偶氮唑盐比色法测定细胞存活率。采用Western blotting和RT-PCR检测E-Cad基因在蛋白和mRNA水平的表达变化。免疫荧光染色和共聚焦荧光显微镜观察E-Cad的表达。结果显示，4HPR处理的T24细胞E-Cad的表达水平显著高于对照组，β-Cat的表达也从胞核向胞浆转移。提示4HPR对膀胱癌T24细胞侵袭的调控作用至少部分是通过调控E-Cad的表达实现的。
Previous researches showed that the expression level of E-Cad in most infiltrating cancer cells was reduced or negative. This study explored whether 4HPR restrained the infiltration of bladder cancer cells through regulating the expression of E-Cad. The infiltrating bladder cancer cells T24 were cultured, and then treated by a proper dosage of drug. Their viability was a determined by MTT method. Western blotting and RT-PCR were adopted to detect the changes of E-Cad gene expression at both protein and mRNA levels. Moreover, immunofluorescent staining and confocal fluorescence microscopy were employed for the observation of the expression of E-Cad. The result showed that, at both mRNA and protein levels, the expression level of E-Cad in T24 cells treated by 4HPR was significantly higher than that of control group, while the β-Cat expression was also relocated from the cell nucleus to cytoplasm. Our findings suggested that the regulatory function of 4HPR on infiltration of bladder cancer cells T24 is at least partly achieved by regulating the expression of E-Cad.
登录
查看更多内容
DOI：
10.1016/j.jsbmb.2007.12.005
发表时间：
2008-03
期刊：
The Journal of Steroid Biochemistry and Molecular Biology
影响因子：
--
作者：
Bin Su;S. M. Mershon;Laura A. Stonerock;R. Curley;R. Brueggemeier
通讯作者：
Bin Su;S. M. Mershon;Laura A. Stonerock;R. Curley;R. Brueggemeier
DOI：
10.1016/s0022-5347(05)64757-x
发表时间：
2002-08
期刊：
The Journal of urology
影响因子：
--
作者：
M. Droller
通讯作者：
M. Droller
影响因子：
11.2
作者：
J. Clifford;D. Menter;Michael Wang;R. Lotan;S. Lippman
通讯作者：
J. Clifford;D. Menter;Michael Wang;R. Lotan;S. Lippman
DOI：
10.3760/cma.j.issn.0529-5807.2010.12.011
发表时间：
2010-12
期刊：
Zhonghua bing li xue za zhi = Chinese journal of pathology
影响因子：
--
作者：
Li-juan Yang;Yu-qin Liu;B. Gu;X. Bian;H. Feng;Zhen-li Yang;Yanyouhong Liu
通讯作者：
Li-juan Yang;Yu-qin Liu;B. Gu;X. Bian;H. Feng;Zhen-li Yang;Yanyouhong Liu
影响因子：
4
作者：
F. Chiesa;N. Tradati;M. Marazza;N. Rossi;P. Boracchi;L. Mariani;F. Formelli;R. Giardini;Alberto Costa;G. D. de Palo;U. Veronesi
通讯作者：
F. Chiesa;N. Tradati;M. Marazza;N. Rossi;P. Boracchi;L. Mariani;F. Formelli;R. Giardini;Alberto Costa;G. D. de Palo;U. Veronesi