TRACE NUTRIENT DEFICIENCY IN DAPHNIA-MAGNA CULTURED IN STANDARD MEDIUM FOR TOXICITY TESTING - EFFECTS OF THE OPTIMIZATION OF CULTURE CONDITIONS ON LIFE-HISTORY PARAMETERS OF DAPHNIA-MAGNA
TRACE NUTRIENT DEFICIENCY IN DAPHNIA-MAGNA CULTURED IN STANDARD MEDIUM FOR TOXICITY TESTING - EFFECTS OF THE OPTIMIZATION OF CULTURE CONDITIONS ON LIFE-HISTORY PARAMETERS OF DAPHNIA-MAGNA
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DOI：
10.1016/0043-1354(90)90180-e
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发表时间：
1990-09-01
期刊：
影响因子：
12.8
通讯作者：
BIAS, WR
中科院分区：
文献类型：
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作者：
ELENDT, BP;BIAS, WR
测试了各种合成培养基对大型溞培养的适用性。在 ISO 提出的用于毒性测试的标准培养基中培养的水蚤表现出繁殖力低、生长速度慢、未发育的孤雌卵数量多以及新生儿和成虫死亡率高。作为一个特征，在 ISO 培养基中培养的动物失去了部分第二触角。可以证明这是由于缺硒引起的。在 ISO 培养基中添加硒明显提高了该培养基的质量。然而，与使用具有复杂微量元素成分的培养基（培养基 M4）相比，多项测试的结果变化更大。迄今为止，培养基 M4 已允许水蚤连续培养三年，没有任何活力或繁殖下降的迹象。根据 19 个非同时实验计算出约 140 个后代/亲代水蚤的平均繁殖率。 M4微量元素浓度增加四倍或减少至十分之一对水蚤的繁殖、生长或存活均没有任何影响。这项研究表明，离子交换柱可以将有害物质浸入基质水中。因此，只能使用超纯基质水来制备培养基。
The suitability of various synthetic media for the culture of Daphnia magna was tested. Daphnids cultured in a standard medium for toxicity testing proposed by ISO showed low reproduction, reduced growth, high numbers of undeveloped parthenogenetic eggs and high mortality of neonates and adults. As a feature, animals cultured in the ISO medium lost parts of their second antennae. It could be shown that this was caused by selenium deficiency. The addition of selenium to the ISO medium clearly improved the quality of this medium. However, results of several tests were more variable than when a medium with a complex trace element composition (medium M4) was used. Medium M4 has allowed continuous culture of daphnids for three years up to now without any signs of reduced viability or reproduction. A mean reproduction rate of about 140 progeny/parent daphnid was calculated from 19 non simultaneous experiments. Neither a fourfold increase nor a reduction to one tenth of the trace element concentration of M4 had any effect on reproduction, growth or survival of daphnids. It was shown in this study that ion-exchanger cartridges can leach detrimental substances into the matrix water. Therefore, only ultrapure matrix waters should be used for the preparation of culture media.