Immediate-early protein of pseudorabies virus is not continuously required to reinitiate transcription of induced genes.
Immediate-early protein of pseudorabies virus is not continuously required to reinitiate transcription of induced genes.
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伪狂犬病病毒的立即早期蛋白并不持续需要重新启动诱导基因的转录。
DOI：
10.1128/jvi.61.4.1258-1260.1987
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发表时间：
1987
影响因子：
5.4
通讯作者：
Feldman,LT
中科院分区：
文献类型：
--
作者：
Ahlers,SE;Feldman,LT
我们利用伪狂犬病病毒即刻早期基因的温度敏感突变体TSG，研究了疱疹病毒即刻早期蛋白在诱导和维持转录中的作用。在允许温度下感染的细胞被转移到非允许温度。最初，两种温度下的早期基因转录速率相似。早期基因转录随后在几个小时内缓慢下降。我们的结果表明，即时早期蛋白功能只对早期基因转录的初始激活是必需的，而不是对激活的早期基因的多次重新启动事件所必需的。
We examined the role of herpesvirus immediate-early proteins in inducing and maintaining transcription by using tsG, a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus. Cells infected at the permissive temperature were shifted to the nonpermissive temperature. Initially, early gene transcription rates were similar at both temperatures. Early gene transcription subsequently decreased slowly over several hours. Our results suggest that immediate-early protein function is required only for the initial activation of early gene transcription and is not required for multiple reinitiation events from activated early genes.