Natriuretic peptides are localized to rat retinal amacrine cells
Natriuretic peptides are localized to rat retinal amacrine cells
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利钠肽定位于大鼠视网膜无长突细胞。
DOI：
10.1016/j.neulet.2007.05.037
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发表时间：
2007-06-27
影响因子：
2.5
通讯作者：
Yang, Xiong-Li
中科院分区：
文献类型：
--
作者：
Jin, Ying;Zhong, Yong-Mei;Yang, Xiong-Li
利钠肽 (NP) 可通过激活三种特定受体亚型 (NPR) 充当神经调节剂。在本研究中，我们通过免疫荧光双标记检测了心房钠尿肽（ANP）、脑钠尿肽（BNP）和C型钠尿肽（CNP）在大鼠不同亚型视网膜无长突细胞（AC）上的表达。所有三种 NP 在多巴胺能和胆碱能 AC 中均适度表达，分别用酪氨酸羟化酶 (TH) 和胆碱乙酰转移酶 (ChAT) 染色。免疫染色出现在这些 AC 的膜、细胞质和体细胞树突区室上。然而，在所有用小清蛋白 (PV) 标记的甘氨酸能 AC 中，仅观察到微弱的点状染色（如果有的话）。这些结果表明，NPs 可以在 ACs 中产生，并在内视网膜中发挥神经调节作用。与之前的免疫细胞化学研究一起，显示 NPR-B 存在于培养的大鼠 GABA 能 AC 中，我们的结果进一步表明 AC 中产生的 NP 也可能调节其自身活性。 (c) 2007 Elsevier Ireland Ltd. 保留所有权利。
Natriuretic peptides (NPs) may act as neuromodulators through activation of three specific receptor subtypes (NPRs). In the present study we examined the expression of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP) on different subtypes of retinal amacrine cells (ACs) in rat by immunofluoresecence double labeling. All three NPs were moderately expressed in dopaminergic and cholinergic ACs, stained by tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT), respectively. The immunostaining appeared on the membrane, cytoplasm and somatodendritic compartments of these ACs. In All glycinergic ACs, labeled by parvalbumin (PV), however, only faint punctate staining, if any, was seen. These results suggest that NPs could be produced in ACs and play a neuromodulatory role in the inner retina. Together with a previous immunocytochemical study, showing that NPR-B is present in cultured rat GABAergic ACs, our results further suggest that NPs produced in ACs may also modulate their own activity. (c) 2007 Elsevier Ireland Ltd. All rights reserved.