Tissue-engineered rabbit cranial suture from autologous fibroblasts and BMP2
Tissue-engineered rabbit cranial suture from autologous fibroblasts and BMP2
复制标题
DOI：
10.1177/154405910408301003
复制
发表时间：
2004-10-01
影响因子：
7.6
通讯作者：
Mao, JJ
中科院分区：
文献类型：
--
作者：
Hong, L;Mao, JJ
颅缝早闭是一种颅缝过早骨化的先天性疾病，大约每2500个活产婴儿中就有一个发生。这项工作解决了一个假设，即颅缝可以从自体细胞组织工程。真皮成纤维细胞从生长的兔子皮下分离，培养扩增，并接种在明胶支架中。我们通过将成纤维细胞接种的明胶支架固定在两个装载有重组人BMP 2的胶原海绵之间来制造复合组织构建体。在获得真皮成纤维细胞的同一只家兔中，用复合组织结构填充手术产生的全层顶骨缺损。在体内植入四周后，组织工程颅缝重新形成，显微镜下使人想起相邻的天然颅缝。组织工程颅缝在放射学图像上显示出射线可透性，与用无成纤维细胞、BMP 2加载的结构填充的显微镜下骨化的颅骨缺损的射线不透性形成对比。这种方法可以改进颅缝早闭患者的颅缝组织工程。
Craniosynostosis is a congenital disorder of premature ossification of cranial sutures, occurring in one of approximately every 2500 live human births. This work addressed a hypothesis that a cranial suture can be tissue-engineered from autologous cells. Dermal fibroblasts were isolated subcutaneously from growing rabbits, culture-expanded, and seeded in a gelatin scaffold. We fabricated a composite tissue construct by sandwiching the fibroblast-seeded gelatin scaffold between two collagen sponges loaded with recombinant human BMP2. Surgically created, full-thickness parietal defects were filled with the composite tissue construct in the same rabbits from which dermal fibroblasts had been obtained. After four-week in vivo implantation, there was de novo formation of tissue-engineered cranial suture, microscopically reminiscent of the adjacent natural cranial suture. The tissue-engineered cranial suture showed radiolucency on radiographic images, in contrast to radio-opacity of microscopically ossified calvarial defects filled with fibroblast-free, BMP2-loaded constructs. This approach may be refined for tissue engineering of cranial sutures for craniosynostosis patients.