A WRKY transcription factor, TaWRKY40‐D, promotes leaf senescence associated with jasmonic acid and abscisic acid pathways in wheat
A WRKY transcription factor, TaWRKY40‐D, promotes leaf senescence associated with jasmonic acid and abscisic acid pathways in wheat
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WRKY 转录因子 TaWRKY40-D 促进小麦叶片衰老，与茉莉酸和脱落酸途径相关
DOI：
10.1111/plb.13155
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发表时间：
2020
期刊：
影响因子：
3.9
通讯作者：
Zhou Chunjiang
中科院分区：
文献类型：
--
作者：
Zhao Ling;Zhang Wenjuan;Song Qiuhang;Xuan Yazhou;Li Ke;Cheng Lingling;Qiao Hualiang;Wang Geng;Zhou Chunjiang
叶片衰老是一个复杂而精确的调控过程，与许多内部和环境因素相关。叶片衰老与养分的重新分配密切相关，这显着影响生产力和质量，尤其是农作物。有证据表明，WRKY 转录因子介导的转录调控对于叶片衰老的微调至关重要。然而，WRKY参与小麦叶片衰老的潜在机制仍不清楚。利用RNA测序数据，我们分离出了一种新的WRKY转录因子TaWRKY40-D，它定位于细胞核，基本上是由叶片衰老的进程诱导的。TaWRKY40-D是转基因拟南芥中自然和黑暗诱导叶片衰老的启动子 拟南芥和小麦。我们还证明了 TaWRKY40-Din 小麦对茉莉酸 (JA) 和脱落酸 (ABA) 处理的积极反应。与此相一致的是，TaWRKY40-DVIGS（病毒诱导基因沉默）小麦植物的分离叶片表现出保持绿色的表型，而表达TaWRKY40-Dover的拟南芥植物在JA和ABA处理后表现出叶片过早衰老。此外，我们的结果表明，TaWRKY40-D 可能通过改变 JA 和 ABA 途径基因的生物合成和信号传导来正向调节叶片衰老。总之，我们的结果表明 JA 和 ABA 相关叶片衰老的新调节因子，以及可用于小麦分子育种的新候选基因。
Leaf senescence is a complex and precise regulatory process that is correlated with numerous internal and environmental factors. Leaf senescence is tightly related to the redistribution of nutrients, which significantly affects productivity and quality, especially in crops. Evidence shows that the mediation of transcriptional regulation by WRKY transcription factors is vital for the fine‐tuning of leaf senescence. However, the underlying mechanisms of the involvement of WRKY in leaf senescence are still unclear in wheat.Using RNA sequencing data, we isolated a novel WRKY transcription factor, TaWRKY40‐D, which localizes in the nucleus and is basically induced by the progression of leaf senescence.TaWRKY40‐D is a promoter of natural and dark‐induced leaf senescence in transgenicArabidopsis thalianaand wheat. We also demonstrated a positive response ofTaWRKY40‐Din wheat upon jasmonic acid (JA) and abscisic acid (ABA) treatment. Consistent with this, the detached leaves ofTaWRKY40‐DVIGS (virus‐induced gene silencing) wheat plants showed a stay‐green phenotype, whileTaWRKY40‐DoverexpressingArabidopsisplants showed premature leaf senescence after JA and ABA treatment. Moreover, our results revealed that TaWRKY40‐D positively regulates leaf senescence, possibly by altering the biosynthesis and signalling of JA and ABA pathway genes.Together, our results suggest a new regulator of JA‐ and ABA‐related leaf senescence, as well as a new candidate gene that can be used for molecular breeding in wheat.