ZCURVE_CoV: a new system to recognize protein coding genes in coronavirus genomes, and its applications in analyzing SARS-CoV genomes.
ZCURVE_CoV: a new system to recognize protein coding genes in coronavirus genomes, and its applications in analyzing SARS-CoV genomes.
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DOI：
10.1016/s0006-291x(03)01192-6
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发表时间：
2003-07-25
影响因子：
3.1
通讯作者：
Zhang CT
中科院分区：
文献类型：
--
作者：
Chen LL;Ou HY;Zhang R;Zhang CT
本文提出了一种识别冠状病毒基因组中蛋白质编码基因的新系统，特别适用于SARS-CoV基因组。与现有的一些系统相比，新的程序包具有简单、准确度高、可靠、快速的优点。 ZCURVE_CoV 系统已针对 11 个新测序的 SARS-CoV 基因组中的每一个进行了运行。因此，对先前未注释的六个基因组进行了注释，并指出并讨论了其余五个基因组中先前注释的一些问题。除了多蛋白链 ORF 1a 和 1b 以及分别编码主要结构蛋白刺突蛋白 (S)、小包膜蛋白 (E)、膜蛋白 (M) 和核壳蛋白 (N) 的四个基因外，ZCURVE_CoV 还预测了 5-6 个长度在 39 到 274 个氨基酸之间的推定蛋白，其功能未知。已经检测到这些推定编码序列内的一些单核苷酸突变，并讨论了它们的生物学意义。提供网络服务，用户将SARS-CoV基因组序列粘贴到网站（http://tubic.tju.edu.cn/sars/）的输入窗口中，即可立即获得注释结果。软件ZCURVE_CoV也可以从上述网址免费下载并在Windows或Linux平台下的计算机上运行。
A new system to recognize protein coding genes in the coronavirus genomes, specially suitable for the SARS-CoV genomes, has been proposed in this paper. Compared with some existing systems, the new program package has the merits of simplicity, high accuracy, reliability, and quickness. The system ZCURVE_CoV has been run for each of the 11 newly sequenced SARS-CoV genomes. Consequently, six genomes not annotated previously have been annotated, and some problems of previous annotations in the remaining five genomes have been pointed out and discussed. In addition to the polyprotein chain ORFs 1a and 1b and the four genes coding for the major structural proteins, spike (S), small envelop (E), membrane (M), and nuleocaspid (N), respectively, ZCURVE_CoV also predicts 5–6 putative proteins in length between 39 and 274 amino acids with unknown functions. Some single nucleotide mutations within these putative coding sequences have been detected and their biological implications are discussed. A web service is provided, by which a user can obtain the annotated result immediately by pasting the SARS-CoV genome sequences into the input window on the web site (http://tubic.tju.edu.cn/sars/). The software ZCURVE_CoV can also be downloaded freely from the web address mentioned above and run in computers under the platforms of Windows or Linux.
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DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
影响因子：
--
作者：
Qin, E;Zhu, QY;Yang, HM
通讯作者：
Yang, HM
影响因子：
14.9
作者：
Besemer, J;Borodovsky, M
通讯作者：
Borodovsky, M
影响因子：
14.9
作者：
Guo, FB;Ou, HY;Zhang, CT
通讯作者：
Zhang, CT
影响因子：
14.9
作者：
THOMPSON, JD;HIGGINS, DG;GIBSON, TJ
通讯作者：
GIBSON, TJ