Breed differences in allele frequency of the dopamine receptor D4 gene in dogs
Breed differences in allele frequency of the dopamine receptor D4 gene in dogs
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DOI：
10.1093/jhered/92.5.433
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发表时间：
2001-09-01
影响因子：
3.1
通讯作者：
Iwasaki, T
中科院分区：
文献类型：
--
作者：
Niimi, Y;Inoue-Murayama, M;Iwasaki, T
我们以前报道过，狗多巴胺受体D4（DRD 4）基因是多态性的，在人类中观察到的，和四个等位基因的数量和/或顺序的12和39 bp的序列位于人类DRD 4的同源区域。为了评估DRD 4基因在犬中的多样性，我们采用聚合酶链反应（PCR）检测了4个品种（比格犬、金毛猎犬、设得兰群岛牧羊犬和柴犬）的等位基因变异。结果，我们发现了三个新的等位基因，并确定了这些等位基因的DNA序列。比格犬共有4个等位基因，包括396、435、447 a和447 b，其中435（52.6%）和447 a（39.5%）等位基因较为常见。金毛犬具有435和447 a等位基因，其中435等位基因频率较高（73.3%）。在设得兰群岛牧羊犬中，观察到435、447 a和498等位基因，其中447 a等位基因频率最高（82.5%）。Shiba有五个等位基因-447 a，447 b，486，498和549-和447 b等位基因是最常见的（55.4%）。这些结果表明，等位基因频率不同的四个狗品种，和DRD 4多态性的分析，因此可能有助于阐明狗品种之间的关系。
We previously reported that the dog dopamine receptor D4 (DRD4) gene is polymorphic as observed in humans, and four alleles were identified based on the number and/or order of the 12 and 39 bp sequences located in the homologous region of human DRD4. To assess the diversity of the DRD4 gene in dogs we examined the allelic variations in four breeds (beagle, golden retriever, Shetland sheepdog, and shiba) employing the polymerase chain reaction (PCR). As a result, we found three novel alleles and determined the DNA sequences of these alleles. The beagle shared four alleles, including 396, 435, 447a, and 447b, with the 435 (52.6%) and 447a (39.5%) alleles being common. The golden retriever had the 435 and 447a alleles, and the 435 allele was frequent (73.3%). In the Shetland sheepdog, the 435, 447a, and 498 alleles were observed, of which the 447a allele was most frequent (82.5%). The shiba had five alleles-447a, 447b, 486, 498, and 549-and the 447b allele was most common (55.4%). These findings suggest that the allele frequency varied among the four dog breeds, and analysis of the DRD4 polymorphism may therefore be useful for elucidating the relationships among dog breeds.