AFM force measurements of the gp120-sCD4 and gp120 or CD4 antigen-antibody interactions
AFM force measurements of the gp120-sCD4 and gp120 or CD4 antigen-antibody interactions
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gp120-sCD4 和 gp120 或 CD4 抗原抗体相互作用的 AFM 力测量
DOI：
10.1016/j.bbrc.2011.03.006
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发表时间：
2011-04-08
影响因子：
3.1
通讯作者：
Chen, Zheng Wei
中科院分区：
文献类型：
--
作者：
Chen, Yong;Zeng, Gucheng;Chen, Zheng Wei
长期以来，可溶性CD_4(SCD_4)、抗CD_4抗体和抗gp120抗体一直被认为是人类免疫缺陷病毒(HIV)治疗的进入抑制物。然而，这些HIV进入抑制剂与相应的靶分子之间的相互作用仍然知之甚少。在本研究中，原子力显微镜(AFM)被用来研究它们之间的相互作用力。我们发现，sCD4-gp120相互作用、CD4抗原-抗体相互作用和gp120抗原-抗体相互作用的解结力分别为25.45+/-20.46、51.22+/-34.64和89.87+/-44.63pN，这可能为理解病毒进入抑制剂对HIV感染的影响提供重要的力学信息。此外，我们发现AFM针尖或衬底上的相互作用对结果有显著的影响，这意味着我们必须更加谨慎地进行AFM力测量和数据分析。(C)2011 Elsevier Inc.保留所有权利。
Soluble CD4 (sCD4), anti-CD4 antibody, and anti-gp120 antibody have long been regarded as entry inhibitors in human immunodeficiency virus (HIV) therapy. However, the interactions between these HIV entry inhibitors and corresponding target molecules are still poorly understood. In this study, atomic force microscopy (AFM) was utilized to investigate the interaction forces among them. We found that the unbinding forces of sCD4-gp120 interaction, CD4 antigen-antibody interaction, and gp120 antigen-antibody interaction were 25.45 +/- 20.46, 51.22 +/- 34.64, and 89.87 +/- 44.63 pN, respectively, which may provide important mechanical information for understanding the effects of viral entry inhibitors on HIV infection. Moreover, we found that the functionalization of an interaction pair on AFM tip or substrate significantly influenced the results, implying that we must perform AFM force measurement and analyze the data with more caution. (C) 2011 Elsevier Inc. All rights reserved.