Potent and selective stimulation of memory-phenotype CD8+ T cells in vivo by IL-15
Potent and selective stimulation of memory-phenotype CD8+ T cells in vivo by IL-15
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DOI：
10.1016/s1074-7613(00)80564-6
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发表时间：
1998-05-01
期刊：
影响因子：
32.4
通讯作者：
Sprent, J
中科院分区：
文献类型：
--
作者：
Zhang, XH;Sun, SQ;Sprent, J
由感染因子诱导的记忆表型（CD 44（hi））CD 8（+）细胞的增殖可以通过注射I型干扰素（IFN I）和IFN I诱导剂（如脂多糖和Poly I：C）来模拟;这种增殖不影响初始T细胞，并且似乎是TCR非依赖性的。由于IFN I抑制体外增殖，IFN I诱导的体内CD 8（+）细胞增殖可能通过产生次级细胞因子间接发生，例如，白细胞介素-2（IL-2）或IL-15。我们在此表明，与IL-2不同，IL-15在体内引起记忆表型CD 44（hi）CD 8（+）（而不是CD 4（+））细胞的强烈和选择性刺激方面与IFN I的作用密切相似;类似的特异性适用于体外纯化的T细胞，并且与CD 8（+）细胞上比CD 4（+）细胞上更高的IL-2 R β表达相关。
Proliferation of memory-phenotype (CD44(hi)) CD8(+) cells induced by infectious agents can be mimicked by injection of type I interferon (IFN I) and by IFN I-inducing agents such as lipopolysaccharide and Poly I:C; such proliferation does not affect naive T cells and appears to be TCR independent. Since IFN I inhibits proliferation in vitro, IFN I-induced proliferation of CD8(+) cells in vivo presumably occurs indirectly through production of secondary cytokines, e.g., interleukin-2 (IL-2) or IL-15. We show here that, unlike IL-2, IL-15 closely mimics the effects of IFN I in causing strong and selective stimulation of memory-phenotype CD44(hi) CD8(+) (but not CD4(+)) cells in vivo; similar specificity applies to purified T cells in vitro and correlates with much higher expression of IL-2R beta on CD8(+) cells than on CD4(+) cells.