A Novel Restraint Device for Injection of Galleria mellonella Larvae that Minimizes the Risk of Accidental Operator Needle Stick Injury
A Novel Restraint Device for Injection of Galleria mellonella Larvae that Minimizes the Risk of Accidental Operator Needle Stick Injury
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DOI：
10.3389/fcimb.2017.00099
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发表时间：
2017-03-28
影响因子：
5.7
通讯作者：
Wiles, Siouxsie
中科院分区：
文献类型：
--
作者：
Dalton, James P.;Uy, Benedict;Wiles, Siouxsie
大蜡螟昆虫的幼虫越来越多地被用于研究病原微生物及其毒力机制，并作为筛选新型抗菌药物的快速模型。幼虫（蜡虫）最常见的感染方式是通过昆虫的前足将病原生物注射到血腔中。最广泛使用的限制注射蜡虫的方法是通过操作者的手指抓住它们，这使操作者面临针刺伤害的风险，这是处理高致病性和/或耐药微生物时的一个重要考虑因素。虽然使用防刺手套可以降低受伤风险，但这样做会损失手动灵活性和速度，导致检测更加劳动密集型和繁琐。我们描述了一种简单且经济有效的装置（所谓的“Galleria Grabber”），用于限制注射蜡虫，使操作者的手指远离针头，从而降低受伤风险。
Larvae of the insect Galleria mellonella are increasingly being used for studying pathogenic microbes and their virulence mechanisms, and as a rapid model for screening novel antimicrobial agents. The larvae (waxworms) are most frequently infected by injection of pathogenic organisms into the haemocoel through the insect's prolegs. The mostly widely used method for restraining the waxworms for injection is by grasping them between the operator's fingers, which puts the operator at risk of needle stick injury, an important consideration when working with highly pathogenic and/or drug-resistant microorganisms. While use of a stab proof glove can reduce this risk of injury, it does so at the loss of manual dexterity and speed, resulting in a more labor-intensive, and cumbersome assay. We describe a simple cost effective device (the so-called "Galleria Grabber") for restraining waxworms for injection that keeps the operator's fingers clear of the needle thus reducing the risk of injury.