Rice MADS6 Interacts with the Floral Homeotic Genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in Specifying Floral Organ Identities and Meristem Fate
Rice MADS6 Interacts with the Floral Homeotic Genes SUPERWOMAN1, MADS3, MADS58, MADS13, and DROOPING LEAF in Specifying Floral Organ Identities and Meristem Fate
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DOI：
10.1105/tpc.111.087262
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发表时间：
2011-07-01
期刊：
影响因子：
11.6
通讯作者：
Zhang, Dabing
中科院分区：
文献类型：
--
作者：
Li, Haifeng;Liang, Wanqi;Zhang, Dabing
AGAMOUS-LIKE 6（AGL 6）基因在花发育中起着重要作用，但它们是否以及如何与花器官身份基因一起工作仍然不太清楚。在这里，我们描述了水稻（Oryza sativa）AGL 6基因MADS 6与其他水稻花同源异型基因在花发育中的相互作用。遗传分析表明，MADS 6与SUPERWOMAN 1/MADS 16（B基因）或MADS 3（C基因）冗余地指定了三个内轮的同一性和花分生组织的决定性。MADS 6通过与MADS 13（D-基因）相互作用来决定心皮/胚珠发育和花的决定性，并与YABBY基因DROOPING LEAF一起控制内稃和花分生组织的特性。表达分析表明，MADS 6 -1中6个B类、C类和E类基因的转录水平在早期花发育阶段降低，表明MADS 6是早期花发育的关键调控因子。此外，MADS 6可以直接结合MADS 58（C基因）上的一个假定的调控基序，并且mads 6 -1 mads 58显示出与mads 6 -1相似的表型。这些结果表明，MADS 6是一个关键的球员在指定花发育通过与其他花同源异型基因在水稻，从而提供了新的见解花发育的控制机制。
AGAMOUS-LIKE6 (AGL6) genes play essential roles in flower development, but whether and how they work with floral organ identity genes remain less understood. Here, we describe interactions of the rice (Oryza sativa) AGL6 gene MADS6 with other rice floral homeotic genes in flower development. Genetic analyses revealed that MADS6 specifies the identity of the three inner whorls and floral meristem determinacy redundantly with SUPERWOMAN1/MADS16 (B-gene) or MADS3 (C-gene). MADS6 was shown to define carpel/ovule development and floral determinacy by interacting with MADS13 (D-gene) and control the palea and floral meristem identities together with the YABBY gene DROOPING LEAF. Expression analyses revealed that the transcript levels of six B-, C-, and E-class genes were reduced in mads6-1 at the early flower developmental stage, suggesting that MADS6 is a key regulator of early flower development. Moreover, MADS6 can directly bind to a putative regulatory motif on MADS58 (C-gene), and mads6-1 mads58 displayed phenotypes similar to that of mads6-1. These results suggest that MADS6 is a key player in specifying flower development via interacting with other floral homeotic genes in rice, thus providing new insights into the mechanism by which flower development is controlled.