Distribution of NPM1-ALK and X-ALK fusion transcripts in paediatric anaplastic large cell lymphoma: a molecular-histological correlation
Distribution of NPM1-ALK and X-ALK fusion transcripts in paediatric anaplastic large cell lymphoma: a molecular-histological correlation
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DOI：
10.1111/j.1365-2141.2009.07754.x
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发表时间：
2009-08-01
影响因子：
6.5
通讯作者：
Woessmann, Willi
中科院分区：
文献类型：
--
作者：
Damm-Welk, Christine;Klapper, Wolfram;Woessmann, Willi
儿童间变性大细胞淋巴瘤（ALCL）表达间变性淋巴瘤激酶（ALK）融合基因，最常见的是NPM 1-ALK。分析了66例儿童ALCL中X-ALK的分布。1例ALCL为ALK阴性。逆转录聚合酶链反应检测NPM 1-ALK在58个肿瘤，所有显示细胞核和细胞质ALK染色。其余7例ALCL仅对细胞质中的ALK进行染色：2例表达TPM 3-ALK，1例表达ATIC-ALK，1例表达MYH 9-ALK; 3例不表达TPM 3-、TFG-、ATIC-、CLTC-或MYH 9-ALK。几乎90%的儿科ALK阳性ALCL表达NPM 1-ALK。ALK染色模式与存在典型/变异ALK融合伴侣之间完全一致。
P>Anaplastic large cell lymphomas (ALCL) in children express anaplastic lymphoma kinase (ALK) fusion genes, most commonly NPM1-ALK. The distribution of X-ALK among 66 childhood ALCLs was analysed. One ALCL was ALK-negative. Reverse transcription polymerase chain reaction detected NPM1-ALK in 58 tumours, all showing nuclear and cytoplasmic ALK staining. The remaining seven ALCL stained for ALK in the cytoplasm only: two expressed TPM3-ALK, one ATIC-ALK, one MYH9-ALK; three no TPM3-, TFG-, ATIC-, CLTC- or MYH9-ALK. Almost 90% of paediatric ALK-positive ALCLs express NPM1-ALK. There was complete concordance between ALK staining pattern and the presence of a typical/variant ALK fusion partner.