IMMUNOCYTOCHEMICAL IDENTIFICATION OF CONE BIPOLAR CELLS IN THE RAT RETINA
IMMUNOCYTOCHEMICAL IDENTIFICATION OF CONE BIPOLAR CELLS IN THE RAT RETINA
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DOI：
10.1002/cne.903610310
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发表时间：
1995-10-23
影响因子：
2.5
通讯作者：
WASSLE, H
中科院分区：
文献类型：
--
作者：
EULER, T;WASSLE, H
我们研究了双极细胞的形态在固定的垂直组织切片的大鼠视网膜注射细胞与荧光黄和神经生物素。根据胞体在内核层中的位置和轴突终末在内丛状层中的分支模式和分层水平，除杆状双极细胞外，还可将9种不同的锥状双极细胞类型区分开来。这些双极细胞群中的一些在垂直和水平切片中使用针对钙结合蛋白recoverin、谷氨酸转运蛋白GLT-1、蛋白激酶C的α亚型和浦肯野细胞标记物L7的抗体进行免疫细胞化学标记。这些免疫细胞化学标记的细胞类型的特点是在细胞密度和distribution.We发现，杆双极细胞和GLT-1阳性锥双极细胞发生在一个小区域位于中央视网膜上部的密度较高。该区域可能对应于中央区域，其是神经节细胞密度最高的区域。第二个高峰的杆双极细胞密度在较低的颞叶周边相匹配的视网膜区域的双眼重叠。免疫细胞化学特征的双极细胞的人口密度表明，至少有50%的双极细胞是锥双极细胞。视锥双极细胞的种类和总数是令人惊讶的，因为大鼠视网膜包含99%的视杆细胞。我们的研究结果表明，锥双极细胞可能发挥更重要的作用，在视觉系统的大鼠比以前认为的。(C)1995年Wiley-Liss，Inc.
We studied the morphology of bipolar cells in fixed vertical tissue sections of the rat retina by injecting the cells with Lucifer Yellow and neurobiotin. In addition to the rod bipolar cell, nine different putative cone bipolar cell, types were distinguished according to the position of their somata in the inner nuclear layer and the branching pattern and stratification level of their axon terminals in the inner plexiform layer. Some of these bipolar cell populations were labeled immunocytochemically in vertical and horizontal sections using antibodies against the calcium-binding protein recoverin, the glutamate transporter GLT-1, the alpha isoform of the protein kinase C, and the Purkinje cell marker L7. These immunocytochemically labeled cell types were characterized in terms of cell density and distribution.We found that rod bipolar cells and GLT-1-positive cone bipolar cells occur at higher densities in a small region located in the upper central retina. This area probably corresponds to the central area, which is the region of highest ganglion cell density. A second peak of rod bipolar cell density in the lower temporal periphery matches the retinal area of binocular overlap. The population densities of the immunocytochemically characterized bipolar cells indicate that at least 50% of all bipolar cells are cone bipolar cells. The variety and total number of cone bipolar cells is surprising because the retina of the rat contains 99% rods. Our findings suggest that cone bipolar cells may play a more important role in the visual system of the rat than previously thought. (C) 1995 Wiley-Liss, Inc.