Expression and secretion of TNF-α in mouse taste buds: a novel function of a specific subset of type II taste cells.
Expression and secretion of TNF-α in mouse taste buds: a novel function of a specific subset of type II taste cells.
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DOI：
10.1371/journal.pone.0043140
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Feng P;Zhao H;Chai J;Huang L;Wang H
味蕾是广泛分布于口腔和喉部表面的化学感觉结构。暴露于口腔环境的味觉细胞在防御潜在病原体方面面临着巨大的挑战。虽然味蕾中很少发现 T 细胞和巨噬细胞等免疫细胞，但在味蕾中观察到一些免疫反应相关分子的高水平表达。然而，这些免疫分子（例如味蕾中的细胞因子）的细胞起源仍有待确定。在这里，我们发现味觉细胞的特定子集选择性地表达高水平的炎症细胞因子肿瘤坏死因子-α（TNF-α）。根据使用味觉细胞类型标记的免疫共定位实验，产生 TNF-α 的细胞主要是表达味觉受体 T1R3 的 II 型味觉细胞。这些细胞可以在体内和体外的炎症挑战下快速增加 TNF-α 的产生和分泌。在TLR2−/−/TLR4−/−双基因敲除小鼠中，脂多糖（LPS）诱导的味蕾细胞中TNF-α的表达被完全消除，这证实了LPS对味蕾中TNF-α的诱导是通过TLR信号通路介导的。味觉细胞产生的 TNF-α 可能有助于局部免疫监视，并在正常和病理条件下调节味觉。
Taste buds are chemosensory structures widely distributed on the surface of the oral cavity and larynx. Taste cells, exposed to the oral environment, face great challenges in defense against potential pathogens. While immune cells, such as T-cells and macrophages, are rarely found in taste buds, high levels of expression of some immune-response-associated molecules are observed in taste buds. Yet, the cellular origins of these immune molecules such as cytokines in taste buds remain to be determined. Here, we show that a specific subset of taste cells selectively expresses high levels of the inflammatory cytokine tumor necrosis factor-α (TNF-α). Based on immuno-colocalization experiments using taste-cell-type markers, the TNF-α-producing cells are predominantly type II taste cells expressing the taste receptor T1R3. These cells can rapidly increase TNF-α production and secretion upon inflammatory challenges, both in vivo and in vitro. The lipopolysaccharide (LPS)-induced TNF-α expression in taste cells was completely eliminated in TLR2−/−/TLR4−/− double-gene-knockout mice, which confirms that the induction of TNF-α in taste buds by LPS is mediated through TLR signaling pathways. The taste-cell-produced TNF-α may contribute to local immune surveillance, as well as regulate taste sensation under normal and pathological conditions.
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影响因子：
4.4
作者：
Nadeau, S;Rivest, S
通讯作者：
Rivest, S
自身免疫性疾病的MRL/LPR小鼠模型中的外周味道结构和功能的缺陷。
影响因子：
3.7
作者：
Kim A;Feng P;Ohkuri T;Sauers D;Cohn ZJ;Chai J;Nelson T;Bachmanov AA;Huang L;Wang H
通讯作者：
Wang H
影响因子：
2.4
作者：
Damak, Sami;Mosinger, Bedrich;Margolskee, Robert F.
通讯作者：
Margolskee, Robert F.
DOI：
10.1016/j.tripleo.2005.05.060
发表时间：
2005-10-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Moen, K;Kvalvik, AG;Brun, JG
通讯作者：
Brun, JG
影响因子：
2.4
作者：
Cohn ZJ;Kim A;Huang L;Brand J;Wang H
通讯作者：
Wang H