Structural characterization of the rat insulin-like growth factor binding protein-6 gene.
Structural characterization of the rat insulin-like growth factor binding protein-6 gene.
复制标题
大鼠胰岛素样生长因子结合蛋白 6 基因的结构特征。
DOI：
10.1006/bbrc.1993.1350
复制
发表时间：
1993
影响因子：
3.1
通讯作者：
Shimasaki,S
中科院分区：
文献类型：
--
作者：
Zhu,X;Ling,N;Shimasaki,S
最近发现了6个不同的胰岛素样生长因子结合蛋白家族，并对前5个家族(L、-2、-3、-4和-5)的基因结构进行了研究。我们现在从大鼠基因组文库中分离了IGFBP-6基因，并确定了它的组织结构以及该基因5‘侧翼区的DNA序列。大鼠IGFBP-6基因全长5.1kb，包含四个外显子，分别由约2.4、0.2和1.2kb的三个内含子打断。利用大鼠肺组织RNA进行的引物延伸分析和核酸酶保护分析表明，ATG翻译起始密码子上游有两个转录起始点，分别位于85和82个核苷酸。大鼠IGFBP-6基因的启动子区域没有TATA或CAAT共有序列基序，但启动子区域存在可能的顺式调控元件，包括Sp1、雌激素受体结合位点和视黄酸反应元件。
Recently a family of six distinct insulin-like growth factor binding proteins (IGFBPs) have been identified and the gene structures of the first five (IGFBP-l, -2, -3, -4 and -5) characterized. We now isolated the IGFBP-6 gene from a rat genomic library and determined its organization as well as the DNA sequence at the 5′ flanking region of the gene. The rat IGFBP-6 gene spans 5.1 kb of the genomic sequence and contains four exons interrupted by three introns of approximately 2.4, 0.2 and 1.2 kb in length, respectively. Primer extension analysis and nbonuclease protection assay using RNA from rat lung tissues demonstrated two transcriptional start sites located at 85 and 82 nucleotides upstream of the ATG translational initiation codon. The promoter region of the rat IGFBP-6 gene is devoid of a TATA or a CAAT consensus sequence motif, but putative regulatory cis elements, including a Sp1, an estrogen receptor binding site and a retinoic acid responsive element, are present in the promoter region.