Regulation of longevity and stress resistance by Sch9 in yeast
Regulation of longevity and stress resistance by Sch9 in yeast
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DOI：
10.1126/science.1059497
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发表时间：
2001-04-13
期刊：
影响因子：
56.9
通讯作者：
Longo, VD
中科院分区：
文献类型：
--
作者：
Fabrizio, P;Pozza, F;Longo, VD
蛋白激酶Akt/蛋白激酶B(PKB)参与哺乳动物的胰岛素信号转导，并通过调节秀丽线虫的寿命和抗逆性发挥作用。我们在不分裂的酿酒酵母中筛选出了长寿突变株，并在腺苷环化酶和SCH9(与Akt/PKB同源)上发现了突变，这些突变可以提高抗氧化剂的能力，使寿命延长三倍。这种寿命的延长需要抗逆转录因子MSN2/MSN4和蛋白激酶Rim15。这些结果表明，寿命与维护投入的增加有关，并表明高度保守的基因在酿酒酵母和高等真核生物的寿命调控中发挥着相似的作用。
The protein kinase Akt/protein kinase B (PKB) is implicated in insulin signaling in mammals and functions in a pathway that regulates Longevity and stress resistance in Caenorhabditis elegans. We screened for Long-Lived mutants in nondividing yeast Saccharomyces cerevisiae and identified mutations in adenylate cyclase and SCH9, which is homologous to Akt/PKB, that increase resistance to oxidants and extend Life-span by up to threefold. Stress-resistance transcription factors Msn2/Msn4 and protein kinase Rim15 were required for this life-span extension. These results indicate that Longevity is associated with increased investment in maintenance and show that highly conserved genes play similar roles in Life-span regulation in S. cerevisiae and higher eukaryotes.